Glycomic Analysis of Tear and Saliva in Ocular Rosacea Patients: The Search for a Biomarker

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dc.contributor.author Vieira, Ana Carolina [UNIFESP]
dc.contributor.author An, Hyun Joo
dc.contributor.author Ozcan, Sureyya
dc.contributor.author Kim, Jae-Han
dc.contributor.author Lebrilla, Carlito B.
dc.contributor.author Mannis, Mark J.
dc.date.accessioned 2018-06-18T11:23:30Z
dc.date.available 2018-06-18T11:23:30Z
dc.date.issued 2012-07-01
dc.identifier http://dx.doi.org/10.1016/j.jtos.2012.04.003
dc.identifier.citation Ocular Surface. New York: Ethis Communicatins, Inc, v. 10, n. 3, p. 184-192, 2012.
dc.identifier.issn 1542-0124
dc.identifier.uri http://repositorio.unifesp.br/11600/45059
dc.description.abstract The purpose of this study was to study changes in glycosylation in tear and saliva obtained from control and ocular rosacea patients in order to identify potential biomarkers for rosacea. Tear fluid was collected from 51 subjects (28 healthy controls and 23 patients with ocular rosacea). Saliva was collected from 42 of the same subjects (25 controls and 17 patients). Pooled and individual samples were examined to determine overall glycan profiles and individual variations in glycosylation. O-and N- glycans were released from both patients and control subjects. Released glycans were purified and enriched by solid-phase extraction (SPE) with graphitized carbon. Glycans were eluted based on glycan size and polarity. SPE fractions were then analyzed by high-resolution mass spectrometry. Glycan compositions were assigned by accurate masses. Their structures were further elucidated by tandem mass spectrometric using collision-induced dissociation (CID), and specific linkage information was obtained by exoglycosidase digestion. N- and O-glycans were released from 20-mu L samples without protein identification, separation, and purification. Approximately 50 N-glycans and 70 O-glycans were globally profiled by mass spectrometry. Most N-glycans were highly fucosylated, while 0-glycans were sulfated. Normal tear fluid and saliva contain highly fucosylated glycans. The numbers of sulfated glycans were dramatically increased in tear and saliva of rosacea patients compared to controls. Glycans found in tear and saliva from roseatic patients present highly quantitative similarity. The abundance of highly fucosylated N-glycans in the control samples and sulfated O-glycans in ocular rosacea patient samples may lead to the discovery of an objective diagnostic marker for the disease. en
dc.description.sponsorship Research to Prevent Blindness, Inc., New York, NY
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent 184-192
dc.language.iso eng
dc.publisher Ethis Communicatins, Inc
dc.relation.ispartof Ocular Surface
dc.rights Acesso restrito
dc.subject biomarkers en
dc.subject fucosylation en
dc.subject glycans en
dc.subject glycomic analysis en
dc.subject mucin en
dc.subject ocular rosacea en
dc.subject oligosaccharides en
dc.subject tear en
dc.subject saliva en
dc.title Glycomic Analysis of Tear and Saliva in Ocular Rosacea Patients: The Search for a Biomarker en
dc.type Artigo
dc.contributor.institution Univ Calif Davis
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ Calif Davis, Dept Ophthalmol, Davis, CA 95616 USA
dc.description.affiliation Univ Fed Sao Paulo, Dept Ophthalmol, Sao Paulo Hosp, Sao Paulo, Brazil
dc.description.affiliation Univ Calif Davis, Dept Chem, Davis, CA 95616 USA
dc.description.affiliation Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA
dc.description.affiliation Univ Calif Davis, Dept Biochem & Mol Med, Davis, CA 95616 USA
dc.description.affiliationUnifesp Univ Fed Sao Paulo, Dept Ophthalmol, Sao Paulo Hosp, Sao Paulo, Brazil
dc.identifier.doi 10.1016/j.jtos.2012.04.003
dc.description.source Web of Science
dc.identifier.wos WOS:000307262200006



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