Anionogenic groups and surface sialoglycoconjugate structures of yeast forms of the human pathogen Paracoccidioides brasiliensis

Anionogenic groups and surface sialoglycoconjugate structures of yeast forms of the human pathogen Paracoccidioides brasiliensis

Author Soares, Regina MA Google Scholar
Silva-Filho, Fernando Costa Google Scholar
Rozental, Sonia Google Scholar
Angluster, Jayme Google Scholar
Souza, Wanderley de Google Scholar
Alviano, Celuta Sales Google Scholar
Travassos, Luiz Rodolpho Autor UNIFESP Google Scholar
Institution Universidade Federal do Rio de Janeiro (UFRJ)
Univ Estadual Norte Fluminense
Universidade Federal de São Paulo (UNIFESP)
Abstract The surface anionogenic groups and sialoglycoconjugate structures of Paracoccidioides brasiliensis yeast forms were analysed by cell microelectrophoresis, binding assays with lectins and viral particles, ultrastructural cytochemistry, enzymic digestion and flow cytofluorimetry. P. brasiliensis yeast forms, particularly the budding primordia, reacted strongly with cationized ferritin. Binding assays showed that the reaction with sialic-acid-specific Limax flavus lectin (LFA) was distributed over the entire P. brasiliensis cell wall. Treatment of yeast forms with neuraminidase significantly reduced their negative surface charge and LFA labelling, which suggests that sialic acid residues are major anionogenic groups exposed on the P. brasiliensis surface. Furthermore, after neuraminidase treatment, labelling with Arachis hypogaea (peanut) agglutinin increased due to unmasking of subterminal beta-D-galactopyranosyl residues. The sialic acid linkages to galactose are alpha 2,6 and alpha 2,3 as assessed, respectively, by fungal attachment to M1/5 and M1/5 HS8 strains of influenza A virus and binding of Sambucus niger and Maackia amurensis agglutinins. The alpha 2,6 linkage clearly predominated in both experiments. Flow cytofluorimetry analysis revealed the heterogenicity of P. brasiliensis yeast cell populations, which comprised young and mature budding yeasts. Both express binding sites to LFA and Limulus polyphemus agglutinin.
Keywords anionogenic groups
influenza virus
Paracoccidioides brasiliensis
sialic acids
yeast forms
Language English
Date 1998-02-01
Published in Microbiology-uk. Reading: Soc General Microbiology, v. 144, n. 2, p. 309-314, 1998.
ISSN 1350-0872 (Sherpa/Romeo, impact factor)
Publisher Soc General Microbiology
Extent 309-314
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000071989700009

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