Adjuvant effect of the propionibacterium acnes and its purified soluble polysaccharide on the immunization with plasmidial DNA containing a Trypanosoma cruzi gene

Adjuvant effect of the propionibacterium acnes and its purified soluble polysaccharide on the immunization with plasmidial DNA containing a Trypanosoma cruzi gene

Author Mussalem, Juliana Sekeres Autor UNIFESP Google Scholar
Vasconcelos, Jose Ronnie Carvalho de Autor UNIFESP Google Scholar
Squaiella, Carla Cristina Autor UNIFESP Google Scholar
Ananias, Renata Zeigler Autor UNIFESP Google Scholar
Braga, Eleni Goncalves Autor UNIFESP Google Scholar
Rodrigues, Mauricio Martins Autor UNIFESP Google Scholar
Longo-Maugeri, Ieda Maria Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract In the present work we investigated the role of killed Propionibacterium acnes or a soluble polysaccharide extracted from bacterium cell wall in modulated experimental immunization with plasmidial DNA. We used a plasmid, p154/13, containing a gene-encoding catalytic domain of Trypanosoma cruzi (T cruzi) trans-sialidase. As previously described, immunization of BALB/c mice with p154/13 elicited Immoral, cell-mediated and protective immune responses against T cruzi infection. In this study we describe that both P acnes and its soluble polysaccharide fraction have the ability to modulate the immune response elicited by p154/13. Treatment with these adjuvants enhanced specific trans-sialidase Th1 immune response, as revealed by a lower IgG1/IgG2a ratio and stronger in vitro IFN-gamma synthesis by CD4(+) T cells. The most important fact was that treatment with P acnes or its soluble polysaccharide fraction in the presence of p154/13 significantly reduced the peak of parasitemia observed 7 to 8 days after T cruzi challenge. These data suggest that P acnes or its soluble polysaccharide fraction may improve the protective potential of a DNA vaccine against experimental T cruzi infection.
Keywords Propionibacterium acnes
immunomodulation
DNA immunization
Trypanosoma cruzi
Language English
Date 2006-01-01
Published in Microbiology And Immunology. Tokyo: Center Academic Publ Japan, v. 50, n. 4, p. 253-263, 2006.
ISSN 0385-5600 (Sherpa/Romeo, impact factor)
Publisher Center Academic Publ Japan
Extent 253-263
Origin http://dx.doi.org/10.1111/j.1348-0421.2006.tb03791.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000236897800001
URI http://repositorio.unifesp.br/11600/44904

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account