Characterization of serotypes and outer membrane protein profiles in enteroaggregative Escherichia coli strains

Characterization of serotypes and outer membrane protein profiles in enteroaggregative Escherichia coli strains

Author Suzart, Sergio Autor UNIFESP Google Scholar
Gomes, Tania Aparecida Tardelli Autor UNIFESP Google Scholar
Guth, Beatriz Ernestina Cabilio Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Twenty-four Escherichia coli strains mainly isolated from children with diarrhea in Sao Paulo, and showing characteristics of enteroaggregative E, coli (EAEC), were characterized by serotyping and outer membrane protein (OMP) profiles. The relationship between these characteristics was evaluated, as well as the usefulness of OMP profiles in the clonal analysis of EAEC strains. All strains presented aggregative adherence to HeLa cells and were classified in two groups based on their interaction with the EAEC DNA probe. A diversity of serotypes and OMP profiles was observed in both groups studied. Although no significant correlation between serotypes and OMP profiles was observed, unique OMP profiles were identified in 80% of the probe-positive strains which were distributed in only 4 OMP profiles. This result may indicate the presence of a few clones in the probe-positive group, On the other hand, probe-negative strains seem to constitute a more diverse group. In general, the observed heterogeneity in serotypes and OMP profiles described in the present study suggest a great genetic diversity in EAEC isolates of either the same or different serotypes and in strains presenting the same EAEC markers identified in our community.
Keywords enteroaggregative Escherichia coli
serotypes
outer membrane protein profiles
Language English
Date 1999-01-01
Published in Microbiology And Immunology. Tokyo: Center Academic Publ Japan, v. 43, n. 3, p. 201-205, 1999.
ISSN 0385-5600 (Sherpa/Romeo, impact factor)
Publisher Center Academic Publ Japan
Extent 201-205
Origin http://dx.doi.org/10.1111/j.1348-0421.1999.tb02394.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000078997300002
URI http://repositorio.unifesp.br/11600/44903

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