The complete amino acid sequence of a trypsin inhibitor from Bauhinia variegata var. candida seeds

The complete amino acid sequence of a trypsin inhibitor from Bauhinia variegata var. candida seeds

Author Di Ciero, Luciana Google Scholar
Oliva, Maria LV Autor UNIFESP Google Scholar
Torquato, Ricardo Autor UNIFESP Google Scholar
Kohler, Peter Google Scholar
Weder, Jürgen KP Google Scholar
Novello, José Camillo Google Scholar
Sampaio, Cláudio AM Autor UNIFESP Google Scholar
Oliveira, Benedito Google Scholar
Marangoni, Sergio Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Tech Univ Munich
Deutsch Forsch Anstalt Lebensmittelchem
Universidade Estadual de Campinas (UNICAMP)
Abstract Trypsin inhibitors of two varieties of Bauhinia variegata seeds have been isolated and characterized. Bauhinia variegata candida trypsin inhibitor (BvcTI) and B. variegata lilac trypsin inhibitor (BVlTI) are proteins with M-r of about 20,000 without free sulfhydryl groups. Amino acid analysis shows a high content of aspartic acid, glutamic acid, serine, and glycine, and a low content of histidine, tyrosine, methionine, and lysine in both inhibitors. Isoelectric focusing for both varieties detected three isoforms (pI 4.85, 5.00, and 5.15), which were resolved by HPLC procedure. The trypsin inhibitors show K-i values of 6.9 and 1.2 nM for BvcTI and BvlTI, respectively. The N-terminal sequences of the three trypsin inhibitor isoforms from both varieties of Bauhinia variegata and the complete amino acid sequence of B. variegata var. candida L. trypsin inhibitor isoform 3 (BvcTI-3) are presented. The sequences have been determined by automated Edman degradation of the reduced and carboxymethylated proteins of the peptides resulting from Staphylococcus aureus protease and trypsin digestion. BvcTI-3 is composed of 167 residues and has a calculated molecular mass of 18,529. Homology studies with other trypsin inhibitors show that BvcTI-3 belongs to the Kunitz family. The putative active site encompasses Arg (63)-Ile (64).
Keywords trypsin inhibitor
Bauhinia variegata seeds
primary structure
sequence homology
Language English
Date 1998-11-01
Published in Journal Of Protein Chemistry. New York: Kluwer Academic/plenum Publ, v. 17, n. 8, p. 827-834, 1998.
ISSN 0277-8033 (Sherpa/Romeo, impact factor)
Publisher Kluwer Academic/plenum Publ
Extent 827-834
Access rights Closed access
Type Article
Web of Science ID WOS:000078226100011

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