Histopathological evaluation of urethroplasty with dorsal buccal mucosa: an experimental study in rabbits

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dc.contributor.author Souza, Geovanne F. [UNIFESP]
dc.contributor.author Calado, Adriano A. [UNIFESP]
dc.contributor.author Delcelo, Rosana [UNIFESP]
dc.contributor.author Ortiz, Valdemar [UNIFESP]
dc.contributor.author Macedo Jr., Antonio [UNIFESP]
dc.date.accessioned 2015-06-14T13:38:37Z
dc.date.available 2015-06-14T13:38:37Z
dc.date.issued 2008-06-01
dc.identifier http://dx.doi.org/10.1590/S1677-55382008000300012
dc.identifier.citation International braz j urol. Sociedade Brasileira de Urologia, v. 34, n. 3, p. 345-354, 2008.
dc.identifier.issn 1677-5538
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/4451
dc.description.abstract PURPOSE: Buccal mucosa is a widely accepted tissue for urethroplasty. The exact healing and tissue integration process, mainly the histological characteristics of dorsal buccal mucosa graft urethroplasty when used dorsally to reconstruct the urethral plate has not previously been assessed, and thus we developed an experimental model to address this question. MATERIALS AND METHODS: In 12 New Zealand rabbits (weight 2.5 kg) we surgically created a dorsal penile urethral defect. A buccal mucosa graft was sutured to the corpora and tunica albuginea, and the ventral urethra anastomosed to this new urethral plate. The animals were divided in three groups and sacrificed 1, 3 and 6 weeks after surgery (groups 1, 2 and 3). A retrograde urethrogram was obtained at autopsy in the last group and the penis analyzed histologically with hematoxylin-eosin and Masson's staining. RESULTS: The urethrograms showed no evidence of fistula or stricture. In group 1 the histopathological analysis showed submucosal lymph-mononuclear inflammatory edema, numerous eosinophils and squamous epithelium integrated into the adjacent urothelium. In group 2 there was no evidence of an inflammatory response but rather complete subepithelial hyaline healing, which was more marked in group 3. CONCLUSION: Healing of buccal mucosa grafts to reconstruct the urethral plate can be achieved by total integration of the squamous epithelium with the urothelium, maintaining the original histological properties of the graft with no fibrosis or retraction. en
dc.format.extent 345-354
dc.language.iso eng
dc.publisher Sociedade Brasileira de Urologia
dc.relation.ispartof International braz j urol
dc.rights Acesso aberto
dc.subject urethra en
dc.subject buccal mucosa en
dc.subject rabbits en
dc.subject experimental en
dc.subject surgery en
dc.title Histopathological evaluation of urethroplasty with dorsal buccal mucosa: an experimental study in rabbits en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Federal University of São Paulo Divisions of Urology
dc.description.affiliation Federal University of São Paulo
dc.description.affiliationUnifesp UNIFESP, Divisions of Urology
dc.description.affiliationUnifesp UNIFESP
dc.identifier.file S1677-55382008000300012.pdf
dc.identifier.scielo S1677-55382008000300012
dc.identifier.doi 10.1590/S1677-55382008000300012
dc.description.source SciELO
dc.identifier.wos WOS:000207704700027


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