Histopathological evaluation of urethroplasty with dorsal buccal mucosa: an experimental study in rabbits

Histopathological evaluation of urethroplasty with dorsal buccal mucosa: an experimental study in rabbits

Autor Souza, Geovanne F. Autor UNIFESP Google Scholar
Calado, Adriano A. Autor UNIFESP Google Scholar
Delcelo, Rosana Autor UNIFESP Google Scholar
Ortiz, Valdemar Autor UNIFESP Google Scholar
Macedo Jr., Antonio Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo PURPOSE: Buccal mucosa is a widely accepted tissue for urethroplasty. The exact healing and tissue integration process, mainly the histological characteristics of dorsal buccal mucosa graft urethroplasty when used dorsally to reconstruct the urethral plate has not previously been assessed, and thus we developed an experimental model to address this question. MATERIALS AND METHODS: In 12 New Zealand rabbits (weight 2.5 kg) we surgically created a dorsal penile urethral defect. A buccal mucosa graft was sutured to the corpora and tunica albuginea, and the ventral urethra anastomosed to this new urethral plate. The animals were divided in three groups and sacrificed 1, 3 and 6 weeks after surgery (groups 1, 2 and 3). A retrograde urethrogram was obtained at autopsy in the last group and the penis analyzed histologically with hematoxylin-eosin and Masson's staining. RESULTS: The urethrograms showed no evidence of fistula or stricture. In group 1 the histopathological analysis showed submucosal lymph-mononuclear inflammatory edema, numerous eosinophils and squamous epithelium integrated into the adjacent urothelium. In group 2 there was no evidence of an inflammatory response but rather complete subepithelial hyaline healing, which was more marked in group 3. CONCLUSION: Healing of buccal mucosa grafts to reconstruct the urethral plate can be achieved by total integration of the squamous epithelium with the urothelium, maintaining the original histological properties of the graft with no fibrosis or retraction.
Palavra-chave urethra
buccal mucosa
rabbits
experimental
surgery
Idioma Inglês
Data de publicação 2008-06-01
Publicado em International braz j urol. Sociedade Brasileira de Urologia, v. 34, n. 3, p. 345-354, 2008.
ISSN 1677-5538 (Sherpa/Romeo, fator de impacto)
Publicador Sociedade Brasileira de Urologia
Extensão 345-354
Fonte http://dx.doi.org/10.1590/S1677-55382008000300012
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000207704700027
SciELO S1677-55382008000300012 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/4451

Exibir registro completo




Arquivo

Nome: S1677-55382008000300012.pdf
Tamanho: 334.4KB
Formato: PDF
Descrição:
Abrir arquivo

Este item está nas seguintes coleções

Buscar


Navegar

Minha conta