Immunochemical and subcellular localization of the 43 kDa glycoprotein antigen of Paracoccidioides brasiliensis with monoclonal antibodies

Immunochemical and subcellular localization of the 43 kDa glycoprotein antigen of Paracoccidioides brasiliensis with monoclonal antibodies

Author Straus, A. H. Google Scholar
Freymuller, E. Google Scholar
Travassos, Luiz Rodolpho Autor UNIFESP Google Scholar
Takahashi, H. K. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Two monoclonal antibodies ST-7 (IgG1) and ST-8 (IgG2b), directed against a 43 kDa glycoprotein (gp43) of Paracoccidioides brasiliensis were produced. It was possible to detect the gp43 by ELISA in amounts as low as 100 ng per well, and by Western blot about 300 ng were detected. Mild treatment of the gp43 with sodium metaperiodate did not alter its reactivity with ST-7 and ST-8, which suggests that these MAbs recognize peptide epitopes. Confirming the periodate oxidation data, the 38 kDa protein resulting from deglycosylation of the gp43 with trifluoromethanesulphonic acid (TMFS), was reactive with ST-7 and ST-8. Immunoelectron microscopy showed that the gp43 is stored inside large dense core vesicles, which flowed into the plasma membrane and extruded from cell membrane into the cell wall. Finally the antigen was secreted into the extracellular space as dense membrane-free material. Secretion of the gp43 occurred at scattered sites interspersed along the cell surface.
Keywords glycoprotein
immunoelectron microscopy
monoclonal antibodies
Paracoccidioides brasiliensis
Language English
Date 1996-06-01
Published in Journal Of Medical And Veterinary Mycology. Oxford: Blackwell Science Ltd, v. 34, n. 3, p. 181-186, 1996.
ISSN 0268-1218 (Sherpa/Romeo, impact factor)
Publisher Blackwell Science Ltd
Extent 181-186
Origin http://dx.doi.org/10.1080/02681219680000301
Access rights Open access Open Access
Type Article
Web of Science ID WOS:A1996UN54900005
URI http://repositorio.unifesp.br/11600/44492

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account