Paracoccidioides brasiliensis-gp43 used as paracoccidioidin

Paracoccidioides brasiliensis-gp43 used as paracoccidioidin

Autor Saraiva, ECO Google Scholar
Altemani, A. Google Scholar
Franco, M. F. Google Scholar
Unterkircher, C. S. Google Scholar
Camargo, Z. P. Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
Universidade de São Paulo (USP)
Resumo A purified glycoprotein of 43 000 daltons from Paracoccidioides brasiliensis (gp43) was tested as paracoccidiodin in delayed-type hypersensitivity (DTH) tests in both experimental animals (guinea pig and mice) and patients with paracoccidioidomycosis (PCM). The gp43 paracoccidioidin was compared with the traditional Fava Netto antigen (AgFN). In guinea pigs, the intradermal injection of 2 mu g of gp43 showed a similar response to those obtained with AgFN, showing in histological sections a population of lymphoid cells that participate in DTH. In mice, gp43 at a dose of 3.75 mu g showed positive DTH response. The use of gp43 as paracoccidioidin in humans showed that this molecule can be used to evaluate the DTH response in patients with PCM. Of 25 PCM patients studied, 48% were positive to gp43 while only 28% were positive to AgFN; 12 PCM patients were completely anergic to both antigens. Considering only those 13 PCM patients who were responsive to gp43 and/or to AgFN, 92.3% reacted against gp43 and 53.8% reacted against AgFN (P < 0.05). Gp43 skin test responses (13.67 +/- 9.56 mm) were significantly larger than those obtained with AgFN (8.43 +/- 3.69 mm). Immunohistochemical study of the human skin showed a perivascular inflammatory response constituted predominantly by T lymphocytes, macrophages and polymorphonuclear leukocytes.
Assunto gp43
Idioma Inglês
Data 1996-06-01
Publicado em Journal Of Medical And Veterinary Mycology. Oxford: Blackwell Science Ltd, v. 34, n. 3, p. 155-161, 1996.
ISSN 0268-1218 (Sherpa/Romeo, fator de impacto)
Editor Blackwell Science Ltd
Extensão 155-161
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:A1996UN54900001

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