The Immunoexpression of Heparanase 2 in Normal Epithelium, Intraepithelial, and Invasive Squamous Neoplasia of the Cervix

The Immunoexpression of Heparanase 2 in Normal Epithelium, Intraepithelial, and Invasive Squamous Neoplasia of the Cervix

Author Marques, Renato Moretti Autor UNIFESP Google Scholar
Focchi, Gustavo Rubino Autor UNIFESP Google Scholar
Theodoro, Therese Rachell Google Scholar
Castelo, Adauto Autor UNIFESP Google Scholar
Pinhal, Maria Aparecida Google Scholar
Nicolau, Sergio Mancini Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Taubate
Abstract Objective. Heparanase 2 (HPSE2) is expressed in various tissues, including the brain, intestine, prostate, breast, and endometrium. The aim of this study was to investigate the role of HPSE2 in cervical carcinogenesis, which has not been clarified to date.Materials and Methods. The immunoexpression of HPSE2 in normal and neoplastic cervical squamous epithelia was determined using a semiquantitative (SQ) method and an index of expression (IE) method, using Image Lab Software. A total of 230 cervical tissue samples were analyzed and segregated into the following diagnostic groups: normal (27.4%), cervical intraepithelial neoplasia 1 (CIN 1, 15.2%), CIN 2 (16.5%), CIN 3(15.2%), and invasive neoplasia (25.7%). The mean HPSE2 expression in the normal group was significantly lower than that of the other groups individually or combined (p < .001, for all combinations). The immunoexpression via the SQ method was significantly greater in the CIN 3 group compared with that in the CIN 1 group (p = .02). The mean immunoexpression of the high-grade squamous intraepithelial lesion groups was significantly greater than those of the normal and low-grade squamous intraepithelial lesion groups (p < .001) and lower compared with that of the invasive neoplasia group (p < .001). There were no statistically significant differences in the immunoexpression of HPSE2 among the different clinical states within the invasive neoplasia group.Conclusions. The SQ method produced a greater sensitivity and specificity than did the index of expression method. There was a progressive increase in the mean HPSE2 immunoexpression according to the severity of the cervical lesion from the low-grade squamous intraepithelial lesion group to the invasive neoplasm group, whereas the normal group displayed the lowest level of expression. This is a novel study concerning HPSE2 in the cervix and cervical cancer carcinogenesis.
Keywords heparanase 2
cervical intraepithelial neoplasia
invasive squamous cell carcinoma
Language English
Date 2012-07-01
Published in Journal Of Lower Genital Tract Disease. Philadelphia: Lippincott Williams & Wilkins, v. 16, n. 3, p. 256-262, 2012.
ISSN 1089-2591 (Sherpa/Romeo, impact factor)
Publisher Lippincott Williams & Wilkins
Extent 256-262
Access rights Closed access
Type Article
Web of Science ID WOS:000305846300008

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