Dimorphic expression of cerebrosides in the mycopathogen Sporothrix schenckii

Dimorphic expression of cerebrosides in the mycopathogen Sporothrix schenckii

Author Toledo, Marcos S. Autor UNIFESP Google Scholar
Levery, Steven B. Google Scholar
Straus, Anita H. Autor UNIFESP Google Scholar
Takahashi, Helio K. Autor UNIFESP Google Scholar
Institution Univ Georgia
Universidade Federal de São Paulo (UNIFESP)
Abstract Major neutral glycosphingolipid components were extracted from Sporothrix schenckii, a dimorphic fungus exhibiting a hyphal saprophytic phase and a yeast parasitic phase responsible for chronic mycotic infections in mammalian hosts. These components, one from the mycelial form and two from the yeast form, were purified and their structures were elucidated by H-1 nuclear magnetic resonance (NMR) spectroscopy, electrospray ionization mass spectrometry (ESI-MS), and tandem ESI-MS/MS, All three were characterized as cerebrosides (monohexosylceramides) containing (4E, 8E)-9-methyl-4,8-sphingadienine as the long-chain base attached to N-2'-hydroxyoctadecanoate and N2'-hydroxy-(E)-Delta(3)-octadecenoate as the fatty acyl components. However, while the mycelial form expressed only beta-glucopyranosylceramide, the yeast form expressed both beta-gluco- and beta-galactopyranosylceramides in approximately equal amounts. In addition, while the glucosylceramides of both mycelial and yeast forms had similar proportions of saturated and (E)-Delta(3) unsaturated 2-hydroxy fatty acid, the galactocerebroside of the yeast form had significantly higher levels of (E)Delta(3) unsaturation. The differences in cerebroside hexose structure represent a novel type of glycosphingolipid dimorphism not previously reported in fungi, Possible implications of these findings with respect to regulation of morphological transitions in S, schenckii and other dimorphic fungi are discussed. Dimorphic expression of cerebrosides in the mycopathogen Sporothrix schenckii.
Keywords electrospray ionization
mass spectrometry
nuclear magnetic resonance spectroscopy
collision-induced dissociation
tandem mass spectrometry
Language English
Date 2000-05-01
Published in Journal Of Lipid Research. Bethesda: Lipid Research Inc, v. 41, n. 5, p. 797-806, 2000.
ISSN 0022-2275 (Sherpa/Romeo, impact factor)
Publisher Lipid Research Inc
Extent 797-806
Origin http://www.jlr.org/content/41/5/797.abstract
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000087054200014
URI http://repositorio.unifesp.br/11600/44472

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