Protective T cell immunity against malaria liver stage after vaccination with live sporozoites under chloroquine treatment

Protective T cell immunity against malaria liver stage after vaccination with live sporozoites under chloroquine treatment

Author Belnoue, Elodie Google Scholar
Costa, Fabio TM Google Scholar
Frankenberg, Tobias Google Scholar
Vigario, Ana Margarida Google Scholar
Voza, Tatiana Google Scholar
Leroy, Nicolas Google Scholar
Rodrigues, Mauricio Martins Autor UNIFESP Google Scholar
Landau, Irène Google Scholar
Snounou, Georges Google Scholar
Renia, Laurent Google Scholar
Institution Univ Paris 05
Universidade Estadual de Campinas (UNICAMP)
Museum Natl Hist Nat
Universidade Federal de São Paulo (UNIFESP)
Inst Pasteur
Abstract In this study we present the first systematic analysis of the immunity induced by normal Plasmodium yoelii sporozoites in mice. Immunization With sporozoites, which was conducted under chloroquine treatment to minimize the influence of blood stage parasites, induced a strong protection against a subsequent sporozoite and, to a lesser extent, against infected RBC challenges. The protection induced by this immunization protocol proved to be very effective. Induction of this protective immunity depended on the presence of liver stage parasites, as primaquine treatment concurrent with sporozoite immunization abrogated protection. Protection was not found to be mediated by the Abs elicited against pre-erythrocytic and blood stage parasites, as demonstrated by inhibition assays of sporozoite penetration or development in vitro and in vivo assays of sporozoite infectivity or blood stage parasite development. CD4(+) and CD8(+) T cells were, however, responsible for the protection through the induction of IFN-gamma and NO. The Journal of Immunology, 2004, 172: 2487-2495.
Language English
Date 2004-02-15
Published in Journal Of Immunology. Bethesda: Amer Assoc Immunologists, v. 172, n. 4, p. 2487-2495, 2004.
ISSN 0022-1767 (Sherpa/Romeo, impact factor)
Publisher Amer Assoc Immunologists
Extent 2487-2495
Origin http://dx.doi.org/10.4049/jimmunol.172.4.2487
Access rights Closed access
Type Article
Web of Science ID WOS:000188788600062
URI http://repositorio.unifesp.br/11600/44416

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