Immunocytochemical localization of heparin in secretory granules of rat peritoneal mast cells using a monoclonal anti-heparin antibody (ST-1)

Immunocytochemical localization of heparin in secretory granules of rat peritoneal mast cells using a monoclonal anti-heparin antibody (ST-1)

Author Oliani, Sonia M. Google Scholar
Freymuller, Edna Autor UNIFESP Google Scholar
Takahashi, Helio K. Autor UNIFESP Google Scholar
Straus, Anita H. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
UNESP
Abstract We performed immunogold labeling with an ST-1 monoclonal antibody (IgM), specific for intact heparin, to define the subcellular localization of heparin in mast cells. Rat peritoneal mast cells were fixed by a modified Karnovsky method and embedded in Araldite. Ultrathin sections were first treated with sodium periodate and then sequentially incubated with MAb ST-1, rabbit anti-mouse IgM, and protein A-gold. By transmission electron microscopy, gold particles were localized inside cytoplasmic granules of peritoneal mast cells. In contrast, with the same procedure, no labeling was observed in mast cells from rat intestinal mucosa. Control sections of rat peritoneal or intestinal mucosa mast Mast cells cells treated with an irrelevant MAb (IgM) did not show any labeling. Treatment with nitrous Heparin acid abolished the reactivity of MAb ST-1 with peritoneal mast cells. These results Granules show that different mast cells can be identified regarding their heparin content by immunochemical procedures using MAb ST-1.
Keywords mast cells
heparin
granules
immunolabeling
monoclonal antibody
electron microscopy
Language English
Date 1997-02-01
Published in Journal Of Histochemistry & Cytochemistry. Seattle: Histochemical Soc Inc, v. 45, n. 2, p. 231-235, 1997.
ISSN 0022-1554 (Sherpa/Romeo, impact factor)
Publisher Histochemical Soc Inc
Extent 231-235
Origin http://dx.doi.org/10.1177/002215549704500208
Access rights Open access Open Access
Type Article
Web of Science ID WOS:A1997WM75700008
URI http://repositorio.unifesp.br/11600/44384

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