Adherence patterns and adherence-related DNA sequences in Escherichia coli isolates from children with and without diarrhea in Sao Paulo city, Brazil

Adherence patterns and adherence-related DNA sequences in Escherichia coli isolates from children with and without diarrhea in Sao Paulo city, Brazil

Author Gomes, Tania Aparecida Tardelli Autor UNIFESP Google Scholar
Vieira, Mônica Aparecida Midolli Autor UNIFESP Google Scholar
Jacob, Cristina Miuki Abe Autor UNIFESP Google Scholar
Rodrigues, Daleth Google Scholar
Griffin, Patricia M. Google Scholar
Ramos, Sonia Regina Testa da Silva Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Ctr Dis Control & Prevent
Abstract The correlation between various adherence patterns and adherence-related DNA sequences in Escherichia coli isolates from 1- to 4-year-old children with and without diarrhea in Sao Paulo, Brazil, was evaluated. A total of 1,801 isolates obtained from 200 patients and 200 age-matched controls were studied. The adherence patterns found were classified as diffuse, aggregative, aggregative in a 6-h assay, aggregative predominantly in coverslips, localized, localized-like, and noncharacteristic. In general, the DNA sequences used as probes showed excellent specificities (>93%), but their sensitivities varied. Thus, the results of bioassays and assays with DNA probes normally used to search for adherent E. coli did not correlate well, and the best method for the identification of these organisms in the clinical research setting remains controversial. Isolates presenting diffuse adherence or hybridizing with the related daaC probe, or both, were by far the most frequent in patients (31.5, 26.0, and 23.0%, respectively), followed by isolates presenting aggregative adherence or hybridizing with the related EAEC probe, or both (21.5, 13.0, and 10.5%, respectively). None of the different combinations of adherence patterns and adherence-related DNA sequences found were associated with acute diarrhea.
Language English
Date 1998-12-01
Published in Journal Of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 36, n. 12, p. 3609-3613, 1998.
ISSN 0095-1137 (Sherpa/Romeo, impact factor)
Publisher Amer Soc Microbiology
Extent 3609-3613
Origin http://jcm.asm.org/content/36/12/3609
Access rights Closed access
Type Article
Web of Science ID WOS:000077069400031
URI http://repositorio.unifesp.br/11600/44300

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