ASSOCIATION BETWEEN ALPHA-HEMOLYSIN PRODUCTION AND HELA CELL-DETACHING ACTIVITY IN FECAL ISOLATES OF ESCHERICHIA-COLI

ASSOCIATION BETWEEN ALPHA-HEMOLYSIN PRODUCTION AND HELA CELL-DETACHING ACTIVITY IN FECAL ISOLATES OF ESCHERICHIA-COLI

Author Marques, Lilian Regina Macelloni Autor UNIFESP Google Scholar
Jacob, Cristina Miuki Abe Autor UNIFESP Google Scholar
Griffin, P. M. Google Scholar
Gomes, Tania Aparecida Tardelli Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
CTR DIS CONTROL & PREVENT
Abstract Escherichia coli isolates that cause detachment of cell monolayers during in vitro adherence assays (cell-detaching E. coli [CDEC]) were recently reported as a potential new group of enteropathogenic bacteria. In the present study, 269 E. coli isolates from feces of children 1 to 5 years of age were identified as CDEC in a detaching assay developed with HeLa cells. The great majority of these isolates,were hemolytic,within 3 h of growth on blood agar plates and hybridized with a DNA probe for alpha-hemolysin (93.7%), while most of the non-detaching isolates were hemolytic,within 24 h (3.6%) or nonhemolytic (94.8%). E. coli isolates that produced alpha hemolysin were found in 60 (30%) of 200 children with diarrhea and 47 (24%) of 200 age-matched controls. No statistical significance was found for the differences in alpha-hemolysin production among the matched pairs (P = 0.2). These data suggest that CDEC isolates are not associated with diarrhea in the population studied.
Language English
Date 1995-10-01
Published in Journal Of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 33, n. 10, p. 2707-2709, 1995.
ISSN 0095-1137 (Sherpa/Romeo, impact factor)
Publisher Amer Soc Microbiology
Extent 2707-2709
Origin http://jcm.asm.org/content/33/10/2707
Access rights Closed access
Type Article
Web of Science ID WOS:A1995RV56500034
URI http://repositorio.unifesp.br/11600/44291

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