DIAGNOSIS OF PARACOCCIDIOIDOMYCOSIS BY DOT-IMMUNOBINDING ASSAY FOR ANTIBODY DETECTION USING THE PURIFIED AND SPECIFIC ANTIGEN GP43

DIAGNOSIS OF PARACOCCIDIOIDOMYCOSIS BY DOT-IMMUNOBINDING ASSAY FOR ANTIBODY DETECTION USING THE PURIFIED AND SPECIFIC ANTIGEN GP43

Author Taborda, Carlos Pelleschi Autor UNIFESP Google Scholar
Camargo, Zoilo Pires de Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The dot immunobinding assay, a rapid, visually read test, was adapted for serodiagnosis and follow-up of paracoccidioidomycosis (PCM). Purified gp43 antigen was tested before and after sodium metaperiodate treatment. To evaluate the assay, it was tested with sera from PCM, histoplasmosis, Jorge Lobo's disease, aspergillosis, candidiasis, and cryptococcosis patients and healthy subjects. Native gp43 gave positive results with all sera from PCM patients and weakly positive results with sera from Jorge Lobo's disease patients (31.3%). No false-positive results were obtained when periodate-treated gp43 was used as the antigen. These results indicate that the dot immunobinding test is sensitive, specific, economical, and fast for serodiagnosis and follow-up studies of PCM.
Language English
Date 1994-02-01
Published in Journal Of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 32, n. 2, p. 554-556, 1994.
ISSN 0095-1137 (Sherpa/Romeo, impact factor)
Publisher Amer Soc Microbiology
Extent 554-556
Origin http://jcm.asm.org/content/32/2/554
Access rights Closed access
Type Article
Web of Science ID WOS:A1994MR79200052
URI http://repositorio.unifesp.br/11600/44290

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