Xenograft Impregnated with Bone Marrow Mononuclear Fraction for Appositional Bone Regeneration in Rabbit Calvaria: A Clinical and Histomorphometric Study

Xenograft Impregnated with Bone Marrow Mononuclear Fraction for Appositional Bone Regeneration in Rabbit Calvaria: A Clinical and Histomorphometric Study

Author Mello e Oliveira, Rafael de Autor UNIFESP Google Scholar
Pelegrine, Andre Antonio Autor UNIFESP Google Scholar
Aloise, Antonio Carlos Autor UNIFESP Google Scholar
Ferreira, Lydia Masako Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Sao Leopoldo Mandic Inst & Res Ctr
Abstract Purpose: This study investigated the combination of a bone marrow mononuclear fraction with a bone xenograft material in an appositional bone regeneration technique. Materials and Methods: Twelve New Zealand rabbits were randomly divided into two groups of six animals each. Bone reconstruction situations were created using titanium cylinders; these were filled with xenograft in group 1 animals and xenograft enriched with bone marrow mononuclear fraction in group 2 animals. Two cylinders were adapted onto the calvaria of each animal. Bone marrow aspirate was obtained from the tibia of every animal. After 8 weeks, the animals were sacrificed and the parietal bone and cylinders were fixed in 10% formalin for analysis of clinical measurement of the bone volume formed inside the cylinders and histomorphometric evaluation of parameters such as vital mineralized tissue (VMT), nonvital mineralized tissue (NVMT), nonmineralized tissue (NMT), and vital mineralized tissue in contact with titanium (VMTCT). Results: Clinically, groups 1 and 2 demonstrated bone volume gains of 88.29% +/- 25.97% and 98.96% +/- 0.00%, respectively. Histomorphometry for groups 1 and 2, respectively, demonstrated the following mean values: VMT, 18.96% +/- 8.99% and 28.02% +/- 8.76%; NVMT, 28.43% +/- 2.44% and 25.57% +/- 2.33%; NMT, 52.61% +/- 10.80% and 46.42% +/- 10.06%; and VMTCT, 4.98% +/- 4.30% and 27.29% +/- 9.58%. Conclusion: The results of this study suggest that the use of the bone marrow mononuclear fraction can improve bone healing and the level of osseointegration.
Keywords bone marrow
bone regeneration
cell transplantation
stromal cells
Language English
Date 2014-07-01
Published in International Journal Of Oral & Maxillofacial Implants. Hanover Park: Quintessence Publishing Co Inc, v. 29, n. 4, p. 962-968, 2014.
ISSN 0882-2786 (Sherpa/Romeo, impact factor)
Publisher Quintessence Publishing Co Inc
Extent 962-968
Origin https://doi.org/10.11607/jomi.3462
Access rights Closed access
Type Article
Web of Science ID WOS:000346119800024
URI http://repositorio.unifesp.br/11600/44093

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