CIRCULATING IMMUNE-COMPLEXES IN LEPROSY SERA - DEMONSTRATION OF ANTIBODIES AGAINST MYCOBACTERIAL GLYCOLIPIDIC ANTIGENS IN ISOLATED IMMUNE-COMPLEXES

CIRCULATING IMMUNE-COMPLEXES IN LEPROSY SERA - DEMONSTRATION OF ANTIBODIES AGAINST MYCOBACTERIAL GLYCOLIPIDIC ANTIGENS IN ISOLATED IMMUNE-COMPLEXES

Author Tomimori, Jane Autor UNIFESP Google Scholar
Cruaud, P. Google Scholar
Papa, F. Google Scholar
Rotta, Osmar Autor UNIFESP Google Scholar
David, H. L. Google Scholar
Institution INST PASTEUR
Universidade Federal de São Paulo (UNIFESP)
HOP JEAN VERDIER
Abstract Circulating immune complexes (CIC) were assayed in sera of leprosy patients. Using an immunoassay for two mycobacterial antigens-phenolic glycolipid-I (PGL-I) and glycolipid IV (SL-IV)-sera from 65 patients with leprosy (38 lepromatous, 18 borderline, and 9 tuberculoid) were studied. The CIC were isolated by polyethylene glycol (PEG) precipitation, washed, treated with an acid buffer, neutralized, and tested using an enzyme-linked immunosorbent assay (ELISA). We demonstrated that CIC could contain IgG and IgM antibodies reacting against PGL-I and SL-IV. The high levels of antibodies in the precipitable CIC showed concordance with high levels in the original sera, although some patients presented high levels of precipitable CIC in the absence of high titers of antibodies in their sera. It was concluded that some of the CIC observed in patients with leprosy were composed of IgG and IgM immunoglobulins against specific mycobacterial antigens.
Language English
Date 1993-03-01
Published in International Journal Of Leprosy And Other Mycobacterial Diseases. Baton Rouge: Amer Leprosy Mission, v. 61, n. 1, p. 44-50, 1993.
ISSN 0148-916X (Sherpa/Romeo, impact factor)
Publisher Amer Leprosy Mission
Extent 44-50
Origin http://www.leprosy-ila.org/leprosyjournal/gn1/detalhe_artigo.php?id=MTA2Mw%3D%3D&secao=ORIGINAL+ARTICLE
Access rights Open access Open Access
Type Article
Web of Science ID WOS:A1993LN70100008
URI http://repositorio.unifesp.br/11600/44051

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