Glycosaminoglycans affect the action of human plasma kallikrein on kininogen hydrolysis and inflammation

Glycosaminoglycans affect the action of human plasma kallikrein on kininogen hydrolysis and inflammation

Author Andrezza, J. G. Google Scholar
Nunes, V. A. Google Scholar
Carmona, Adriana Karaoglanovic Autor UNIFESP Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Dietrich, Carl Peter Autor UNIFESP Google Scholar
Silveira, Vera Lucia Flor Autor UNIFESP Google Scholar
Shimamoto, K. Google Scholar
Ura, N. Google Scholar
Sampaio, Misako Uemura Autor UNIFESP Google Scholar
Sampaio, Claudio Augusto Machado Autor UNIFESP Google Scholar
Araujo, M. S. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Sapporo Med Univ
Abstract Human plasma kallikrein (huPK) is a serine proteinase involved in many biological processes including those of the kallikrein-kinin system. The action of huPK on kininogen results in bradykinin (BK) release, a potent mediator of inflammatory responses. BK generation may be influenced by several agents, and the aim of this work was to investigate the effect of glycosaminoglycans (GAGs) on human high-molecular-weight kininogen (HK) hydrolysis by huPK and on inflammation.huPK was pre-incubated in the absence and presence of different GAGs, followed by the addition of kininogen. Bradykinin released at different times was measured by radioimmunoassay, and K-M and k(cat) were calculated. Tuna and bovine dermatan sulfates, the most potent GAGs studied, reduced by 80% and 68%. respectively, the catalytic efficiency of huPK (control=4.1 x 10(4) M-1 s(-1)) in BK release.The effect of bovine dermatan sulfate (BDS) on inflammatory response was studied in rat paw edema induced by carrageenin and hourly determined (1-4 h) by plethysmography. BDS significantly reduced the inflammatory response in the first and second hours of measurements (24% and 28%, respectively), p < 0.05.GAGs were shown to reduce bradykinin release in vitro and in an inflammation model. This reduction may play a role in the control or maintenance of some pathological and physiological processes. (C) 2002 Published by Elsevier Science B.V.
Keywords human plasma kallikrein
high-molecular-weight kininogen
paw edema
Language English
Date 2002-12-01
Published in International Immunopharmacology. Amsterdam: Elsevier Science Bv, v. 2, n. 13-14, p. 1861-1865, 2002.
ISSN 1567-5769 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 1861-1865
Access rights Closed access
Type Article
Web of Science ID WOS:000179753500017

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