Systemic Host Response Following Skin Burn Injury in Rats: Cytotoxicity and Genotoxicity Evaluation

Systemic Host Response Following Skin Burn Injury in Rats: Cytotoxicity and Genotoxicity Evaluation

Author Quintana, Hananiah Tardivo Autor UNIFESP Google Scholar
Bortolin, Jeferson Andre Autor UNIFESP Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Oliveira, Flavia de Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Aim: The aim of the present study was to investigate whether skin burn injury (BI) can induce cellular changes in skeletal muscle, liver, kidney and blood by means of DNA damage and cytotoxicity. Materials and Methods: Thirty male Wistar rats were distributed into two groups: control (C) and submitted to scald burn (SB), subdivided into three subgroups: 1, 4 or 14 days post-injury. The gastrocnemius muscle and liver were dissected for histopathological evaluation and the single-cell gel (comet) assay was used to investigate damage in skeletal muscle, liver, kidney and blood cells. Results: Histopathological analysis of the muscle in the SB group revealed congested vessels containing inflammatory cells for all periods evaluated post-injury. In liver, the one day post-injury SB group showed sinusoidal congestion, while that of 14 days post-injury exhibited an increased number of Kupffer cells. Conclusion: Despite the histophatological evidence, none of the groups showed any signs of genotoxicity in these target tissues.
Keywords genetic damage
rat
burn injury
histopathology
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number FAPESP: 11/22034-9
Date 2014-11-01
Published in In Vivo. Athens: Int Inst Anticancer Research, v. 28, n. 6, p. 1065-1070, 2014.
ISSN 0258-851X (Sherpa/Romeo, impact factor)
Publisher Int Inst Anticancer Research
Extent 1065-1070
Origin http://iv.iiarjournals.org/content/28/6/1065.full
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000345225400008
URI http://repositorio.unifesp.br/11600/43919

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