Cell-detaching Escherichia coli (CDEC) strains from children with diarrhea: identification of a protein with toxigenic activity

Cell-detaching Escherichia coli (CDEC) strains from children with diarrhea: identification of a protein with toxigenic activity

Author Fabrega, VLA Google Scholar
Ferreira, AJP Google Scholar
Patricio, Francy Reis da Silva Autor UNIFESP Google Scholar
Brinkley, C. Google Scholar
Scaletsky, Isabel Cristina Affonso Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Univ Maryland
Abstract Twenty-four strains of cell-detaching Escherichia coli (CDEC) isolated from stool specimens in different cities in Brazil were examined for virulence properties. Aerobactin production and multiple antibiotic resistance were observed in most of the isolates. In hybridization studies, the ahly, pap, and cnf sequences, common properties of this category of E. coli, were found in a minority of isolates. Half of the CDEC isolates had enteroaggregative DNA sequences (pet, astA, aggA), six strains carried the shet1 gene, nine strains carried the daaC sequence, and one strain carried the stp gene. Thirteen strains induced fluid accumulation in the rabbit intestinal loop assay. Supernatant filtrate of one of those strains, which did not hybridize with any of the toxin probes tested, induced destructive lesions in the rabbit ileal loop and enterotoxic activity in the Ussing chamber. A 12-kDa protein purified by 60% ammonium sulfate precipitation of the supernatant filtrate demonstrated a toxigenic effect that was inhibited by the anti-12-kDa protein antiserum. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Keywords cell-detaching Escherichia coli
virulence property
diarrhea
toxin
Language English
Date 2002-12-17
Published in Fems Microbiology Letters. Amsterdam: Elsevier Science Bv, v. 217, n. 2, p. 191-197, 2002.
ISSN 0378-1097 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 191-197
Origin http://www.sciencedirect.com/science/article/pii/S0378109702010716
Access rights Closed access
Type Article
Web of Science ID WOS:000179884500009
URI http://repositorio.unifesp.br/11600/43678

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