Effect of bauhinia bauhinioides kallikrein inhibitor on endothelial proliferation and intracellular calcium concentration

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dc.contributor.author Bilgin, M.
dc.contributor.author Burgazli, K. M.
dc.contributor.author Rafiq, A.
dc.contributor.author Mericliler, M.
dc.contributor.author Neuhof, C.
dc.contributor.author Oliva, M. L. [UNIFESP]
dc.contributor.author Parahuleva, M.
dc.contributor.author Soydan, N.
dc.contributor.author Doerr, O.
dc.contributor.author Abdallah, Y.
dc.contributor.author Erdogan, A.
dc.date.accessioned 2018-06-15T17:22:30Z
dc.date.available 2018-06-15T17:22:30Z
dc.date.issued 2014-01-01
dc.identifier http://www.europeanreview.org/article/6456
dc.identifier.citation European Review For Medical And Pharmacological Sciences. Rome: Verduci Publisher, v. 18, n. 1, p. 46-51, 2014.
dc.identifier.issn 1128-3602
dc.identifier.uri http://repositorio.unifesp.br/11600/43630
dc.description.abstract OBJECTIVES: Proteinase inhibitors act as a defensive system against predators e.g. insects, in plants. Bauhinia bauhinioides kallikrein inhibitor (BbKI) is a serine proteinase inhibitor, isolated from seeds of Bauhinia bauhinioides and is structurally similar to plant Kunitz-type inhibitors but lacks disulfide bridges. In this study we evaluated the antiproliferative effect of BbKI on endothelial cells and its impact on changes in membrane potential and intracellular calcium.MATERIALS AND METHODS: HUVEC proliferation was significantly reduced by incubation with BbKI 50 and 100 mu M 12% and 13%. Furthermore, BbKI (100 mu M) exposure caused a significant increase in intracellular Ca2+ concentration by 35% as compared to untreated control.RESULTS: The intracellular rise in calcium was not affected by the absence of extracellular calcium. BBKI also caused a significant change in the cell membrane potential but the antiproliferative effect was independent of changes in membrane potential.CONCLUSIONS: BBKI has an antiproliferative effect on HUVEC, which is independent of the changes in membrane potential, and it causes an increase in intracellular Ca2+. en
dc.format.extent 46-51
dc.language.iso eng
dc.publisher Verduci Publisher
dc.relation.ispartof European Review For Medical And Pharmacological Sciences
dc.rights Acesso aberto
dc.subject Kallikrein inhibitor en
dc.subject Angiogenesis en
dc.subject Endothelial cells en
dc.subject Calcium en
dc.subject Hyperpolarization en
dc.subject Proliferation en
dc.title Effect of bauhinia bauhinioides kallikrein inhibitor on endothelial proliferation and intracellular calcium concentration en
dc.type Artigo
dc.contributor.institution Univ Giessen
dc.contributor.institution Bezmialem Vakif Univ
dc.contributor.institution Wuppertal Res & Med Ctr
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ Giessen, Dept Cardiol & Angiol, D-35390 Giessen, Germany
dc.description.affiliation Bezmialem Vakif Univ, Fac Med, Dept Radiol, Istanbul, Turkey
dc.description.affiliation Wuppertal Res & Med Ctr, Dept Internal Med & Angiol, Wuppertal, Germany
dc.description.affiliation Univ Fed Sao Paulo, Dept Biochem, Sao Paulo, Brazil
dc.description.affiliationUnifesp Univ Fed Sao Paulo, Dept Biochem, Sao Paulo, Brazil
dc.description.source Web of Science
dc.identifier.wos WOS:000331436700006


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