Effect of bauhinia bauhinioides kallikrein inhibitor on endothelial proliferation and intracellular calcium concentration

Effect of bauhinia bauhinioides kallikrein inhibitor on endothelial proliferation and intracellular calcium concentration

Author Bilgin, M. Google Scholar
Burgazli, K. M. Google Scholar
Rafiq, A. Google Scholar
Mericliler, M. Google Scholar
Neuhof, C. Google Scholar
Oliva, M. L. Autor UNIFESP Google Scholar
Parahuleva, M. Google Scholar
Soydan, N. Google Scholar
Doerr, O. Google Scholar
Abdallah, Y. Google Scholar
Erdogan, A. Google Scholar
Institution Univ Giessen
Bezmialem Vakif Univ
Wuppertal Res & Med Ctr
Universidade Federal de São Paulo (UNIFESP)
Abstract OBJECTIVES: Proteinase inhibitors act as a defensive system against predators e.g. insects, in plants. Bauhinia bauhinioides kallikrein inhibitor (BbKI) is a serine proteinase inhibitor, isolated from seeds of Bauhinia bauhinioides and is structurally similar to plant Kunitz-type inhibitors but lacks disulfide bridges. In this study we evaluated the antiproliferative effect of BbKI on endothelial cells and its impact on changes in membrane potential and intracellular calcium.MATERIALS AND METHODS: HUVEC proliferation was significantly reduced by incubation with BbKI 50 and 100 mu M 12% and 13%. Furthermore, BbKI (100 mu M) exposure caused a significant increase in intracellular Ca2+ concentration by 35% as compared to untreated control.RESULTS: The intracellular rise in calcium was not affected by the absence of extracellular calcium. BBKI also caused a significant change in the cell membrane potential but the antiproliferative effect was independent of changes in membrane potential.CONCLUSIONS: BBKI has an antiproliferative effect on HUVEC, which is independent of the changes in membrane potential, and it causes an increase in intracellular Ca2+.
Keywords Kallikrein inhibitor
Angiogenesis
Endothelial cells
Calcium
Hyperpolarization
Proliferation
Language English
Date 2014-01-01
Published in European Review For Medical And Pharmacological Sciences. Rome: Verduci Publisher, v. 18, n. 1, p. 46-51, 2014.
ISSN 1128-3602 (Sherpa/Romeo, impact factor)
Publisher Verduci Publisher
Extent 46-51
Origin http://www.europeanreview.org/article/6456
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000331436700006
URI http://repositorio.unifesp.br/11600/43630

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