Prognostic markers of low-grade squamous intraepithelial lesions: the role of topoisomerase II alpha and active caspase-3

Prognostic markers of low-grade squamous intraepithelial lesions: the role of topoisomerase II alpha and active caspase-3

Author Coelho, Raquel Autran Autor UNIFESP Google Scholar
Focchi, Gustavo Rubino de Azevedo Autor UNIFESP Google Scholar
Nogueira-de-Souza, Naiara Corrêa Autor UNIFESP Google Scholar
Sartori, Marair Gracio Ferreira Autor UNIFESP Google Scholar
Silva, Ismael Dale Cotrim Guerreiro da Autor UNIFESP Google Scholar
Ribalta, Julisa Chamorro Lascasas Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Purpose: To study the relationship between topoisomerase II alpha, active caspase-3 expressions and HPV DNA in uterine cervices with low-grade squamous intraepithelial lesions (LSIL). Methods: Forty women with LSIL and 32 without cervical neoplasia diagnosed through cytologic and histopathologic examination were evaluated regarding topoisomerase II alpha and active caspase-3 expressions and HPV DNA detection using PCR (GP5/GP6) in cervicovaginal smears. Results: The mean percentage of cells immunomarked by topoisomerase in the group with LSIL was 11.62% while in the control it was 4.13% (p < 0.0001). In the presence of HPV DNA, topoisomerase expression was higher in the group With productive viral infection than in nonneoplastic tissue (p = 0.004). Caspase-3 expression was observed in 17 patients with LSIL (42.5%) and in five without cervical neoplasia (15.63%). Conclusion: The use of topoisomerase II alpha and active caspase-3 in cervical biopsies may help to define the prognosis of HPV cervical infection.
Keywords topoisomerase II alpha
caspase-3
HPV
neoplasia
Language English
Date 2008-01-01
Published in European Journal Of Gynaecological Oncology. Montreal: I R O G Canada, Inc, v. 29, n. 5, p. 499-501, 2008.
ISSN 0392-2936 (Sherpa/Romeo, impact factor)
Publisher I R O G Canada, Inc
Extent 499-501
Origin http://www.irog.net/download/?magazine=91
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000259328800017
URI http://repositorio.unifesp.br/11600/43599

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