Heparanase-2, syndecan-1, and extracellular matrix remodeling in colorectal carcinoma

Heparanase-2, syndecan-1, and extracellular matrix remodeling in colorectal carcinoma

Author Peretti, Thais Autor UNIFESP Google Scholar
Waisberg, Jaques Autor UNIFESP Google Scholar
Mader, Ana Maria Amaral Antonio Autor UNIFESP Google Scholar
Matos, Leandro Luongo de Autor UNIFESP Google Scholar
Costa, Ricardo B. da Google Scholar
Conceição, Gleice Margarete de Souza Autor UNIFESP Google Scholar
Lopes, Antonio Carlos Autor UNIFESP Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Pinhal, Maria Aparecida da Silva Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Hosp Servidor Publ Estadual
Hosp Estadual Mario Covas
Fdn ABC
Abstract Aim To propose a quantitative method to detect heparanase-2 (HPA2) and syndecan-1 (Syn-1) using immunohistochemistry in colorectal (colon and rectal) carcinomas compared with nonneoplastic tissues and evaluate the possible role of these molecules in tumor development and extracellular remodeling.Methods Cytoplasmic staining of HPA2 and Syn-1 was obtained by standard immunohistochemical reactions in 50 colorectal carcinoma and 20 nonneoplastic large bowels tissues. An image system was used to quantity the immunoexpression by digital computer-assisted method (Matos et al. 2006). The cutoff point for the immunohistochemistry variable was defined by sensibility and specificity curves. Statistical analysis was performed using SPSS version 13.0.Results HPA2 was over-expressed in colorectal cancer (131.1 +/- 24.9 o.u./mu m(2)) when compared with nonneoplastic tissues (27.9 +/- 12.2 o.u./mu m(2)) (P<0.0001). However, an opposite correlation was observed between Syn-1 and tumor presence, where colorectal tissues expressed lower Syn-1 proteoglycan compared with nonneoplastic tissues, respectively (39.2 +/- 17.8 o.u./mu m(2)) and (102.2 +/- 25.2 o.u./mu m(2)) (P<0.0001).Conclusion A methodology with high sensitivity and specificity is proposed with a cutoff value for HPA2 and Syn-1 in the immunohistochemistry assay to define the presence of tumor. It was demonstrated for the first time in the literature that HPA2 is over-expressed in colorectal carcinoma tissues compared with nonneoplastic tissues. HPA2 over-expression could be possibly related to Syn-1 shedding despite the fact that HPA2 does not present enzymatic activity as HPA1.
Keywords colorectal carcinoma
heparanase-2
immunohistochemistry
proteoglycans
syndecan-1
Language English
Date 2008-08-01
Published in European Journal Of Gastroenterology & Hepatology. Philadelphia: Lippincott Williams & Wilkins, v. 20, n. 8, p. 756-765, 2008.
ISSN 0954-691X (Sherpa/Romeo, impact factor)
Publisher Lippincott Williams & Wilkins
Extent 756-765
Origin https://doi.org/10.1097/MEG.0b013e3282fc2649
Access rights Closed access
Type Article
Web of Science ID WOS:000257829000008
URI http://repositorio.unifesp.br/11600/43589

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