Affected and non-affected skin fibroblasts from systemic sclerosis patients share a gene expression profile deviated from the one observed in healthy individuals

Affected and non-affected skin fibroblasts from systemic sclerosis patients share a gene expression profile deviated from the one observed in healthy individuals

Author Fuzii, Hellen Thais Autor UNIFESP Google Scholar
Yoshikawa, Gilberto Toshimitsu Autor UNIFESP Google Scholar
Junta, C. M. Google Scholar
Sandrin-Garcia, P. Google Scholar
Fachin, A. L. Google Scholar
Sakamoto-Hojo, E. T. Google Scholar
Donadi, E. A. Google Scholar
Passos, G. A. S. Google Scholar
Andrade, Luiz Eduardo Coelho Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract ObjectivesTo evaluate the gene expression profile of fibroblasts from affected and non-affected skin of systemic sclerosis (SSc) patients and from controls.Materials and methodsLabeled cDNA from fibroblast cultures from forearm (affected) and axillary (non-affected) skin from six diffuse SSc patients, from three normal controls, and from MOLT-4/HEp-2/normal fibroblasts (reference pool) was probed in microarrays generated with 4193 human cDNAs from the IMAGE Consortium. Microarray images were converted into numerical data and gene expression was calculated as the ratio between fibroblast cDNA (Cy5) and reference pool cDNA (Cy3) data and analyzed by R environment/Aroma, Cluster, Tree View, and SAM softwares. Differential expression was confirmed by real time PCR for a set of selected genes.ResultsEighty-eight genes were up- and 241 genes down-regulated in SSc fibroblasts. Gene expression correlation was strong between affected and non-affected fibroblast samples from the same patient (r>0.8), moderate among fibroblasts from all patients (r=0.72) and among fibroblasts from all controls (r=0.70), and modest among fibroblasts from patients and controls (r=0.55). The differential expression was confirmed by real time PCR for all selected genes.ConclusionsFibroblasts from affected and non-affected skin of SSc patients shared a similar abnormal gene expression profile, suggesting that the widespread molecular disturbance in SSc fibroblasts is more sensitive than histological and clinical alterations. Novel molecular elements potentially involved in SSc pathogenesis were identified.
Keywords Systemic sclerosis
gene expression
fibroblast
cDNA microarrays
Language English
Date 2008-09-01
Published in Clinical And Experimental Rheumatology. Pisa: Clinical & Exper Rheumatology, v. 26, n. 5, p. 866-874, 2008.
ISSN 0392-856X (Sherpa/Romeo, impact factor)
Publisher Clinical & Exper Rheumatology
Extent 866-874
Origin http://www.clinexprheumatol.org/article.asp?a=3499
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000261248600017
URI http://repositorio.unifesp.br/11600/43317

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