Evidence of idiotypic modulation in the immune response to gp43, the major antigenic component of Paracoccidioides brasiliensis in both mice and humans

Evidence of idiotypic modulation in the immune response to gp43, the major antigenic component of Paracoccidioides brasiliensis in both mice and humans

Author Souza, A. R. Google Scholar
Gesztesi, Jean-Luc Autor UNIFESP Google Scholar
Moraes, Jane Zveiter de Autor UNIFESP Google Scholar
Cruz, CRB Google Scholar
Sato, J. Google Scholar
Mariano, Mario Autor UNIFESP Google Scholar
Lopes, Jose Daniel Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Paracoccidioidomycosis (PCM) is a systemic mycosis endemic in Latin America, with a high prevalence in Brazil, Argentina, Colombia and Venezuela. The aetiologic agent of disease is a thermal dimorphic fungus, Paracoccidioides brasiliensis. A glycoprotein of 43 000D (gp43) is the major antigen of P. brasiliensis. Antibodies directed to this antigen are detected in the sera of all patients with PCM. Gp43 binds to laminin, thus participating in adhesion, invasion and pathogenesis of the fungus. As the role of antibodies in PCM is not fully understood, we decided to investigate the outcome of mice immunization with three distinct anti-gp43 MoAbs (17c, 8a and 24a) coupled with keyhole limpet haemocyanin (KLH). Results show not only the expected presence of anti Id (AB2) antibodies in the sera of these animals but also a spontaneous and increasing amount of anti-anti-Id (AB3) antibodies after the third course of immunization. Hybridomas producing both AB2 and AB3 MoAbs were obtained using spleen cells from mice immunized with MoAb 17c. AB3 MoAbs were also obtained with spleen cells of mice immunized with MoAbs 8a and 24a. It was also shown that human PCM patients' sera with high titres of anti-gp43 antibodies generate anti-Id antibodies. These data suggest that the immune response to P. brasiliensis can be spontaneously modulated by the idiotypic network.
Keywords Paracoccidioides brasiliensis
gp43
idiotypic network
monoclonal antibodies
human infection
Language English
Date 1998-10-01
Published in Clinical And Experimental Immunology. Oxford: Blackwell Science Ltd, v. 114, n. 1, p. 40-48, 1998.
ISSN 0009-9104 (Sherpa/Romeo, impact factor)
Publisher Blackwell Science Ltd
Extent 40-48
Origin http://dx.doi.org/10.1111/j.1365-2249.2008.03767.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000076271900007
URI http://repositorio.unifesp.br/11600/43282

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