GP43 from Paracoccidioides brasiliensis inhibits macrophage functions. An evasion mechanism of the fungus

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dc.contributor.author Popi, Ana Flavia [UNIFESP]
dc.contributor.author Lopes, Jose Daniel [UNIFESP]
dc.contributor.author Mariano, Mario [UNIFESP]
dc.date.accessioned 2018-06-15T16:39:16Z
dc.date.available 2018-06-15T16:39:16Z
dc.date.issued 2002-07-01
dc.identifier http://dx.doi.org/10.1016/S0008-8749(02)00576-2
dc.identifier.citation Cellular Immunology. San Diego: Academic Press Inc Elsevier Science, v. 218, n. 1-2, p. 87-94, 2002.
dc.identifier.issn 0008-8749
dc.identifier.uri http://repositorio.unifesp.br/11600/43260
dc.description.abstract Macrophages constitute one of the primary cellular mechanisms that impairs parasite invasion of host tissues. The phagocytic and microbicidal properties of these cells can be modulated by specific membrane receptors involved in cell-microorganism interactions. Gp43, the main antigen secreted by Paracoccidiodes brasiliensis (Pb), the causative agent of Paracoccidioidomycosis, is a high mannose glycoprotein. The role played by gp43 in the pathogenesis of the disease is not completely known. Here, we describe the influence of this molecule on the interaction between peritoneal murine macrophages and Pb. Phagocytosis of Pb, live or heat-killed, by adherent peritoneal cells from both, B10.A (susceptible) and A/Sn (resistant) mice, was evaluated. Addition of different concentrations of gp43 to the culture medium inhibited, in a dose-dependent pattern, phagocytosis of live or heat-killed Pb by peritoneal macrophages from both B10.A and A/Sn mice. Gp43 also inhibits phagocytosis of zymosan particles but did not interfere with the uptake of opsonized sheep red blood cells. It was also shown that both gp43 and heat-killed Pb have an inhibitory effect on the release of NO by zymosan stimulated macrophages. Finally, we demonstrated that gp43 inhibits the fungicidal ability of macrophages from both lineages. Based on these data, it is suggested that gp43 can be considered one of the evasion mechanisms for the installation of primary infection in susceptible hosts. (C) 2002 Elsevier Science (USA). All rights reserved. en
dc.format.extent 87-94
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Cellular Immunology
dc.rights Acesso restrito
dc.subject Paracoccidiodes brasiliensis en
dc.subject gp43 en
dc.subject macrophages en
dc.subject phagocytosis en
dc.subject microbicidal activity en
dc.subject escape mechanism en
dc.title GP43 from Paracoccidioides brasiliensis inhibits macrophage functions. An evasion mechanism of the fungus en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ Fed Sao Paulo, Discipline Immunol, Dept Microbiol Immunol & Parasitol, Sao Paulo, Brazil
dc.description.affiliationUnifesp Univ Fed Sao Paulo, Discipline Immunol, Dept Microbiol Immunol & Parasitol, Sao Paulo, Brazil
dc.identifier.doi 10.1016/S0008-8749(02)00576-2
dc.description.source Web of Science
dc.identifier.wos WOS:000179962300009



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