Structure of heparan sulfate: Identification of variable and constant oligosaccharide domains in eight heparan sulfates of different origins

Structure of heparan sulfate: Identification of variable and constant oligosaccharide domains in eight heparan sulfates of different origins

Author Dietrich, Carl Peter Autor UNIFESP Google Scholar
Tersariol, Ivarne Luis dos Santos Autor UNIFESP Google Scholar
Toma, Leny Autor UNIFESP Google Scholar
Moraes, Carlos Torres Autor UNIFESP Google Scholar
Porcionatto, Marimélia Aparecida Autor UNIFESP Google Scholar
Oliveira, Fernanda Wanderley de Autor UNIFESP Google Scholar
Nader, Helena Bonciani Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The use of specific enzymes (heparinase and heparitinases from Flavobncterium heparinum, endoglucuronidase, alpha N-acetylglucosaminidase and beta-glucuronidase from the mollusc Anomalocardia brasiliana) and chemical methods (nitrous acid degradation, hydrazine N-deacetylation and borohydride reduction), led to the proposal of the total sequence of a heparan sulfate derived from bovine pancreas and partial sequences of heparan sulfates from different origins (bovine: lung, liver, brain; hog: liver, brain; rabbit liver; dog liver). It was shown that all the heparan sulfates contain common structural features such as: a N-acetylated and a N-sulfated domain made of glucuronic acid-containing disaccharides and a more sulfated region made of iduronic acid-containing disaccharides. Separating the two domains a peculiar tetrasaccharide made of GlcNAc-(alpha 1-4)-IdoUA-(alpha 1-4)-GlcNS-(alpha 1-4)-IdoUA was identified in all the heparan sulfates analyzed. It was also shown that the non-reducing ends of the heparan sulfates contain the monosaccharides glucosamine N-sulfate and/or glucosamine 2,6 disulfate.
Keywords heparan sulfate, structure
heparan sulfate, disaccharide sequence
heparan sulfates, structural similarities
heparan sulfate, oligosaccharide domains
Language English
Date 1998-05-01
Published in Cellular And Molecular Biology. Noisy-le-grand: Cellular & Molecular Biology, v. 44, n. 3, p. 417-429, 1998.
ISSN 0145-5680 (Sherpa/Romeo, impact factor)
Publisher Cellular & Molecular Biology
Extent 417-429
Access rights Closed access
Type Article
Web of Science ID WOS:000074915900004
URI http://repositorio.unifesp.br/11600/43259

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