Immunohistochemical profile of lymphoid lesions of the orbit

Immunohistochemical profile of lymphoid lesions of the orbit

Author Lowen, Marcia Serva Autor UNIFESP Google Scholar
Saraiva, V. S. Google Scholar
Martins, M. C. Google Scholar
Burnier Júnior, Miguel Noel Nascente Autor UNIFESP Google Scholar
Institution McGill Univ
Universidade Federal de São Paulo (UNIFESP)
Abstract Background: Orbital idiopathic inflammation, lymphoid hyperplasia, and lymphoma may all present clinically in the same manner. Histopathology and especially immunohistochemistry play a major role in the differential diagnosis. The purpose of this study was to determine the immunophenotypic features of these lesions.Methods: Fifty-five orbital lymphoid lesions were retrieved from the ophthalmic pathology registries at McGill University, Montreal, Canada, and the Federal University of Sao Paulo, Sao Paulo, Brazil. Formalin-fixed, paraffin-embedded, histopathologic sections were stained with hematoxylin and eosin and periodic acid-Schiff. The sections were also immunostained for B-cell (CD20) and T-cell (CD43) markers and for immunoglobulin light chains kappa and lambda. Two pathologists determined the histopathologic and immunohistochemical pattern of each lesion in a masked fashion.Results: Of the 55 lesions, 11 (20%) were idiopathic chronic inflammations, 22 (40%) were lymphoid hyperplasias and 22 (40%) were lymphomas. Idiopathic inflammation displayed a predominance of T cells and all lesions expressed polyclonal light chains. Lymphoid hyperplasia displayed a mixture of B cells and T cells, with a slight predominance of the former and all lesions expressed polyclonal light chains. Lymphoma showed a striking predominance of B cells and all lesions expressed monoclonal light chains, usually kappa (63.7%). The differences in the mean percentages of B cells among the orbital lymphoid lesions (inflammation, 35%; hyperplasia, 65.9%; lymphoma, 87.3%) were statistically significant (p < 0.001).Interpretation: Orbital lymphoid lesions can be differentiated based on the percentages of B cells and T cells and the monoclonal or polyclonal expression of immunoglobulin light chains.
Keywords lymphoproliferative disorders
Language English
Date 2005-10-01
Published in Canadian Journal Of Ophthalmology-journal Canadien D Ophtalmologie. Ottawa: Canadian Ophthal Soc, v. 40, n. 5, p. 634-639, 2005.
ISSN 0008-4182 (Sherpa/Romeo, impact factor)
Publisher Canadian Ophthal Soc
Extent 634-639
Access rights Closed access
Type Article
Web of Science ID WOS:000233178600015

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