Evaluation of normal ocular bacterial flora with two different culture media

Evaluation of normal ocular bacterial flora with two different culture media

Author Moeller, Cecilia Tobias de Aguiar Autor UNIFESP Google Scholar
Branco, Bruno Castelo Autor UNIFESP Google Scholar
Yu, Maria Cecília Zorat Autor UNIFESP Google Scholar
Farah, Michel Eid Autor UNIFESP Google Scholar
Santos, MAA Google Scholar
Hofling-Lima, Ana Luisa Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Background: The purpose of this study was to determine if the use of broth culture medium is efficient in investigating bacterial flora of the normal eyelid and conjunctiva.Methods: Samples from the conjunctiva and eyelid of healthy patients of various ages who were undergoing ocular surgeries were obtained and cultured at 3 periods: before topical antibiotic prophylaxis, in the postoperative period during topical antibiotic treatment, and 15 days after discontinuation of antibiotic use. Samples were inoculated into both brain heart infusion broth and blood agar plate, and the growth results of both media were analyzed.Results: Brain heart infusion broth medium showed a significantly higher bacterial growth of gram-positive cocci in most periods.The solid blood agar medium had a higher recovery of gram-positive bacilli before prophylaxis only in the older patients.Interpretation: Our results show that a more complete analysis of eyelid and conjunctival flora can be obtained using both liquid and solid media to increase the chances of isolate recovery. The inclusion of liquid media in this analysis was even more relevant in the period of concomitant use of antibiotic treatment.
Keywords conjunctiva
flora
bacteria
culture
brain heart infusion
agar
Language English
Date 2005-08-01
Published in Canadian Journal Of Ophthalmology-journal Canadien D Ophtalmologie. Ottawa: Canadian Ophthal Soc, v. 40, n. 4, p. 448-453, 2005.
ISSN 0008-4182 (Sherpa/Romeo, impact factor)
Publisher Canadian Ophthal Soc
Extent 448-453
Origin http://dx.doi.org/10.1016/S0008-4182(05)80004-3
Access rights Closed access
Type Article
Web of Science ID WOS:000231848400004
URI http://repositorio.unifesp.br/11600/43228

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