Immunohistochemical expression of S-100 beta in choroidal melanomas

Immunohistochemical expression of S-100 beta in choroidal melanomas

Author Martins, M. C. Google Scholar
Scull, J. J. Google Scholar
Alcocer, C. E. Google Scholar
Deschenes, J. Google Scholar
Antecka, E. Google Scholar
Burnier Júnior, Miguel Noel Nascente Autor UNIFESP Google Scholar
Institution MCGILL UNIV
Universidade Federal de São Paulo (UNIFESP)
Abstract Objective: To investigate the expressivity of S-100 beta antibodies in choroidal melanomas and to compare it with that of S-100 protein and HMB-45.Design: Twenty-seven choroidal melanoma specimens obtained from the McGill University Ophthalmic Pathology Registry were classified as spindle cell, epithelioid cell or mixed-cell type. Immunohistochemistry was performed using the standard peroxidase-antiperoxidase technique with monoclonal HMB-45, polyclonal S-100, polyclonal S-100 beta and monoclonal S-100 alpha beta antibodies in formalin-fixed, paraffin-embedded sections.Outcome measure: Intensity of immunoreaction. The result was considered positive when at least five focal areas of stained cells were observed within the tumour.Results: All 27 tumours were positive for HMB-45, 19 (70%) for S-100, 23 (85%) for S-100 beta, and 21 (78%) for S-100 alpha beta. No correlation was found between the intensity of the immunoreaction and cell classification,Conclusions: HMB-45 is the most reliable marker for choroidal melanomas, S-100 beta is a more sensitive marker than S-100 for choroidal melanomas regardless of cell type. Contrary to previous reports, S-100 beta should not be considered a useful immunomarker to differentiate between primary choroidal melanoma and cutaneous melanoma metastatic to the choroid.
Keywords choroidal melanomas
S-100 protein
S-100 beta
Language English
Date 1997-10-01
Published in Canadian Journal Of Ophthalmology-journal Canadien D Ophtalmologie. Ottawa: Canadian Ophthal Soc, v. 32, n. 6, p. 378-381, 1997.
ISSN 0008-4182 (Sherpa/Romeo, impact factor)
Publisher Canadian Ophthal Soc
Extent 378-381
Access rights Closed access
Type Article
Web of Science ID WOS:A1997YE82000003

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