Comparison of colony and stool blots for detection of enteropathogens by dna probes

Comparison of colony and stool blots for detection of enteropathogens by dna probes

Author Vieira, MAM Autor UNIFESP Google Scholar
Guth, Beatriz Ernestina Cabilio Autor UNIFESP Google Scholar
Gomes, Tania Aparecida Tardelli Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract DNA probes that identify genes coding for heat-labile type I (LT-I) and heat-stable type 1 (ST-I) enterotoxins, enteropathogenic Escherichia coli adherence factor (EAF), and Shigella-like invasiveness (INV) are used to evaluate the sensitivity and specificity of stool blots in comparison with the sensitivity and specificity of colony blots in detecting enteropathoghens. The sensitivities of the probes in stool blots are 91.7% for the LT-I probe, 76.9% for the ST-I probe, 76.9% for the ST-I probes, 78.9% for the EAF probe, and 45.5% for the INV probe. The specificity of all probes is higher than 95%. In general, the stool blot method identifies as many if not more LT-I-, ST-I-, and EAF-producing E. coli infections than the colony blots.
Keywords DNA PROBES
STOOL BLOTS
ENTEROPATHOGENS
DIAGNOSIS
Language English
Date 1991-05-01
Published in Canadian Journal Of Microbiology. Ottawa: Natl Research Council Canada, v. 37, n. 5, p. 407-410, 1991.
ISSN 0008-4166 (Sherpa/Romeo, impact factor)
Publisher Natl Research Council Canada
Extent 407-410
Origin http://www.nrcresearchpress.com/doi/abs/10.1139/m91-066#.WEFVatLys1I
Access rights Closed access
Type Article
Web of Science ID WOS:A1991FT81600014
URI http://repositorio.unifesp.br/11600/43219

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