COMPARISON OF OUTER-MEMBRANE PROTEIN AND LIPOPOLYSACCHARIDE PROFILES OF ENTEROTOXIGENIC ESCHERICHIA-COLI STRAINS ISOLATED IN SAO-PAULO, BRAZIL

COMPARISON OF OUTER-MEMBRANE PROTEIN AND LIPOPOLYSACCHARIDE PROFILES OF ENTEROTOXIGENIC ESCHERICHIA-COLI STRAINS ISOLATED IN SAO-PAULO, BRAZIL

Author Guth, Beatriz Ernestina Cabilio Autor UNIFESP Google Scholar
Pacheco, ABF Google Scholar
Vonkruger, W. M. Google Scholar
Ferreira, LCS Google Scholar
Institution Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Federal de São Paulo (UNIFESP)
Abstract The outer membrane protein (OMP) and lipopolysaccharide (LPS) patterns of 12 strains of serogroups of enterotoxigenic E. coli frequently isolated in Sao Paulo city were determined by fractionation techniques and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Five O6, three O78 and four O128 serogroup isolates of different serotypes (flagellar anti) and virulence factors (toxins and colonization factor antigens) showed a high degree of variability in their OMP pattern and at least nine groups could be identified. The analysis of LPS patterns by SDS-PAGE showed a homogeneous profile for the O6 strains and some minor differences for the O128 and O78 strains. The present data indicate that analysis of OMP and LPS by SDS-PAGE may further improve the discriminating ability of extensively used serological techniques or the detection of virulence factors and could be a useful tool in epidemiological studies of enterotoxigenic E. coli (ETEC) strains from this area.
Keywords ETEC
ESCHERICHIA COLI
OUTER MEMBRANE PROTEINS
LIPOPOLYSACCHARIDES
Language English
Date 1995-05-01
Published in Brazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 28, n. 5, p. 545-552, 1995.
ISSN 0100-879X (Sherpa/Romeo, impact factor)
Publisher Assoc Bras Divulg Cientifica
Extent 545-552
Access rights Closed access
Type Article
Web of Science ID WOS:A1995RG22200006
URI http://repositorio.unifesp.br/11600/43084

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