O-Glycosidically linked n-acetylglucosamine-bound oligosaccharides from glycoproteins of Trypanosoma-Cruzi

O-Glycosidically linked n-acetylglucosamine-bound oligosaccharides from glycoproteins of Trypanosoma-Cruzi

Author Previato, José O. Google Scholar
Jones, Christopher Google Scholar
Goncalves, Luciana PB Google Scholar
Wait, Robin Google Scholar
Travassos, Luiz Rodolpho Autor UNIFESP Google Scholar
Mendonca-Previato, Lucia Google Scholar
Institution NATL INST BIOL STAND & CONTROLS
Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Federal de São Paulo (UNIFESP)
PUBL HLTH LAB SERV
Abstract In this report we describe studies on the structures of the O-linked carbohydrate units in cell-surface glycoproteins of epimastigote forms of the G-strain of Trypanosoma cruzi. Mild alkaline reductive degradation of the 38/43 kDa glycoproteins resulted in beta-elimination of glycosylated threonine and/or serine residues, and the liberation of N-acetylglucosaminitol, galactobiosyl-, galactotriosyl-, galactotetraosyl and galacto-pentaosyl-N-acetylglucosaminitol. The structures of these oligosaccharide alditols were established by n.m.r. spectroscopy and methylation analysis as: Galf beta 1-4(Galp beta 1-6)GlcNAc-ol Galp beta 1-3Galp beta 1-6(Galf beta 1-4)GlcNAc-ol; [(Galp beta 1-3)(Galp beta 1-2)Galp beta 1-6](Galf beta 1-4)GlcNAc-ol; [(Galp beta 1-3)(Galp beta 1-2)Galp beta 1-6](Galp beta 1 -2Galf beta 1-4)GlcNAc-ol.
Language English
Date 1994-07-01
Published in Biochemical Journal. London: Portland Press, v. 301, n. 1, p. 151-159, 1994.
ISSN 0264-6021 (Sherpa/Romeo, impact factor)
Publisher Portland Press
Extent 151-159
Origin http://dx.doi.org/10.1042/bj3010151
Access rights Open access Open Access
Type Article
Web of Science ID WOS:A1994NY66100024
URI http://repositorio.unifesp.br/11600/42794

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