DNA mismatch repair gene methylation in gastric cancer in individuals from northern Brazil

DNA mismatch repair gene methylation in gastric cancer in individuals from northern Brazil

Author Lima, Eleonidas Moura Google Scholar
Leal, Mariana Ferreira Autor UNIFESP Google Scholar
Smith, Marilia de Arruda Cardoso Autor UNIFESP Google Scholar
Burbano, Rommel Rodríguez Autor UNIFESP Google Scholar
Assumpcao, Paulo Pimentel Autor UNIFESP Google Scholar
Bello, Maria Jose Google Scholar
Rey, Juan Antonio Google Scholar
Lima, Francinaldo Ferreira de Google Scholar
Casartelli, Cacilda Google Scholar
Institution Univ Fed Piaui
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Fed Univ Para
Hosp Joao Barros Barreto
Inst Invest Biomed
Abstract Gastric cancer is one of the most common malignancies. DNA methylation is implicated in DNA mismatch repair genes deficiency. In the present study, we evaluated the methylation status of MLH1, MSH2, MSH6 and PMS2 in 20 diffuse- and 26 intestinal-type gastric cancer samples and 20 normal gastric mucosal of gastric cancer patients from Northern Brazil. We found that none of the nonneoplastic samples showed methylation of any gene promoter and 50% of gastric, cancer samples showed at least one methylated gene promoter. Methylation frequencies of MLH1, MSH2, MSH6 and PMS2 promoter were 21.74%, 17.39%, 0% and 28.26% respectively in gastric cancer samples. MLH1 and PMS2 methylation were associated with neoplastic samples compared to nonneoplastic ones. PMS2:? methylation was associated with diffuse- and intestinal-type cancer compared with normal controls. Intestinal-type cancer showed significant association with MLH1 methylation. Diffuse-type cancer was significantly associated with MSH2 methylation. Our findings show differential gene methylation in tumoral tissue, which allows us to conclude that methylation is associated with gastric carcinogenesis. Methylation of mismatch repair genes was associated with gastric carcinogenesis and may be a helpful tool for diagnosis, prognosis and therapies. However, MSH6 does not seem to be regulated by methylation in our samples.
Keywords gastric cancer
MLH1
MSH2
MSH6
PMS2
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
FAEPA
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Financiadora de Estudos a Projetos
Grant number Financiadora de Estudos a Projetos: FINEP/CT-INFRA 0927-03
Date 2008-12-01
Published in Biocell. Mendoza: Inst Histol Embriol-conicet, v. 32, n. 3, p. 237-243, 2008.
ISSN 0327-9545 (Sherpa/Romeo, impact factor)
Publisher Inst Histol Embriol-conicet
Extent 237-243
Origin http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000300004&lng=en&nrm=iso&tlng=en
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000262866400004
SciELO ID S0327-95452008000300004 (statistics in SciELO)
URI http://repositorio.unifesp.br/11600/42783

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