Erythrocyte Protoporphyrin Fluorescence as a Potential Marker of Diabetes

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dc.contributor.author Fauaz, Grasiele [UNIFESP]
dc.contributor.author Miranda, Adriana Regina
dc.contributor.author Gomes, Cinthia Zanini
dc.contributor.author Courrol, Lilia Coronato [UNIFESP]
dc.contributor.author Silva, Flavia Rodrigues de Oliveira [UNIFESP]
dc.contributor.author Rocha, Flavia Gomes de Goes [UNIFESP]
dc.contributor.author Schor, Nestor [UNIFESP]
dc.contributor.author Bellini, Maria Helena [UNIFESP]
dc.date.accessioned 2018-06-15T13:55:57Z
dc.date.available 2018-06-15T13:55:57Z
dc.date.issued 2010-04-01
dc.identifier http://dx.doi.org/10.1366/000370210791114248
dc.identifier.citation Applied Spectroscopy. Frederick: Soc Applied Spectroscopy, v. 64, n. 4, p. 391-395, 2010.
dc.identifier.issn 0003-7028
dc.identifier.uri http://repositorio.unifesp.br/11600/42618
dc.description.abstract Protoporphyrin (PpIX), a porphyrin derivative, is the intermediate metabolic precursor of the heme molecule. Abnormal metabolism of total erythrocyte PpIX has been observed in diseases such as cancer, lead poisoning, psoriasis, iron deficiency anemia and acute porphyries. Diabetes mellitus (DM) is a complex metabolic syndrome in which hyperglycemia is the primary clinical manifestation and contributes to the diabetic complications. The aim of this study was to evaluate the utility of fluorescence spectroscopy of erythrocyte PpIX for monitoring the early stages of diabetes. A total of 14 male C 57BL mice, 6 weeks old, were divided into two groups: diabetic and non-diabetic. Diabetes was induced by intraperitoneal injection of streptozotocin (SZT). Blood cells were cultured with standard and 50 mM supplemented RPMI medium. Blood smears were prepared and stained for qualitative morphology analysis under optical microscopy. Blood porphyrin autofluorescence was analyzed by fluorescence spectroscopy. Characteristic PpIX emission spectra were obtained by exciting the samples at 405 nm. Average blood glucose was lower in the control group than in the diabetic group (156.50 +/- 8.11 mg/dL vs. 371.10 +/- 14.43 mg/dL P < 0.05). Both diabetic and glucose-cultured erythroblasts showed a significant decrease (around 30.5% and 40%, respectively) in the emission band intensity at 635 nm. Our results indicate that the erythrocyte PpIX profile could be used as a biological monitor for diabetes. en
dc.description.sponsorship Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent 391-395
dc.language.iso eng
dc.publisher Soc Applied Spectroscopy
dc.relation.ispartof Applied Spectroscopy
dc.rights Acesso restrito
dc.subject Diabetes en
dc.subject Porphyrin en
dc.subject Fluorescence en
dc.subject Autofluorescence spectroscopy en
dc.subject Animal model en
dc.title Erythrocyte Protoporphyrin Fluorescence as a Potential Marker of Diabetes en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution IPEN CNEN SP
dc.description.affiliation Univ Fed Sao Paulo, Dept Med, Disciplina Nefrol, Sao Paulo, Brazil
dc.description.affiliation IPEN CNEN SP, Ctr Biotecnol, Sao Paulo, Brazil
dc.description.affiliation Univ Fed Sao Paulo, Dept Ciencias Exatas & Terra, Sao Paulo, Brazil
dc.description.affiliation IPEN CNEN SP, Ctr Lasers & Aplicacoes, Sao Paulo, Brazil
dc.description.affiliationUnifesp Univ Fed Sao Paulo, Dept Med, Disciplina Nefrol, Sao Paulo, Brazil
dc.description.affiliationUnifesp Univ Fed Sao Paulo, Dept Ciencias Exatas & Terra, Sao Paulo, Brazil
dc.description.sponsorshipID FAPESP: 03/54253-6
dc.description.sponsorshipID CNPq: 481888/2008
dc.identifier.doi 10.1366/000370210791114248
dc.description.source Web of Science
dc.identifier.wos WOS:000277001200006



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