Erythrocyte Protoporphyrin Fluorescence as a Potential Marker of Diabetes

Erythrocyte Protoporphyrin Fluorescence as a Potential Marker of Diabetes

Author Fauaz, Grasiele Autor UNIFESP Google Scholar
Miranda, Adriana Regina Google Scholar
Gomes, Cinthia Zanini Google Scholar
Courrol, Lilia Coronato Autor UNIFESP Google Scholar
Silva, Flavia Rodrigues de Oliveira Autor UNIFESP Google Scholar
Rocha, Flavia Gomes de Goes Autor UNIFESP Google Scholar
Schor, Nestor Autor UNIFESP Google Scholar
Bellini, Maria Helena Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
IPEN CNEN SP
Abstract Protoporphyrin (PpIX), a porphyrin derivative, is the intermediate metabolic precursor of the heme molecule. Abnormal metabolism of total erythrocyte PpIX has been observed in diseases such as cancer, lead poisoning, psoriasis, iron deficiency anemia and acute porphyries. Diabetes mellitus (DM) is a complex metabolic syndrome in which hyperglycemia is the primary clinical manifestation and contributes to the diabetic complications. The aim of this study was to evaluate the utility of fluorescence spectroscopy of erythrocyte PpIX for monitoring the early stages of diabetes. A total of 14 male C 57BL mice, 6 weeks old, were divided into two groups: diabetic and non-diabetic. Diabetes was induced by intraperitoneal injection of streptozotocin (SZT). Blood cells were cultured with standard and 50 mM supplemented RPMI medium. Blood smears were prepared and stained for qualitative morphology analysis under optical microscopy. Blood porphyrin autofluorescence was analyzed by fluorescence spectroscopy. Characteristic PpIX emission spectra were obtained by exciting the samples at 405 nm. Average blood glucose was lower in the control group than in the diabetic group (156.50 +/- 8.11 mg/dL vs. 371.10 +/- 14.43 mg/dL P < 0.05). Both diabetic and glucose-cultured erythroblasts showed a significant decrease (around 30.5% and 40%, respectively) in the emission band intensity at 635 nm. Our results indicate that the erythrocyte PpIX profile could be used as a biological monitor for diabetes.
Keywords Diabetes
Porphyrin
Fluorescence
Autofluorescence spectroscopy
Animal model
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 03/54253-6
CNPq: 481888/2008
Date 2010-04-01
Published in Applied Spectroscopy. Frederick: Soc Applied Spectroscopy, v. 64, n. 4, p. 391-395, 2010.
ISSN 0003-7028 (Sherpa/Romeo, impact factor)
Publisher Soc Applied Spectroscopy
Extent 391-395
Origin http://dx.doi.org/10.1366/000370210791114248
Access rights Closed access
Type Article
Web of Science ID WOS:000277001200006
URI http://repositorio.unifesp.br/11600/42618

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