Amifostine Increases FAS and Caspase-3 Expression in Colonic Tissue of Irradiated Mice

Amifostine Increases FAS and Caspase-3 Expression in Colonic Tissue of Irradiated Mice

Autor Oshima, Celina Tizuko Fujiyama Autor UNIFESP Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Gomes, Thiago Simao Autor UNIFESP Google Scholar
Adios, Priscila C. Autor UNIFESP Google Scholar
Egami, Mizue Imoto Autor UNIFESP Google Scholar
Segreto, Helena Regina Comodo Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Resumo Background/Aim: The aim of this study was to evaluate the expression of FASL, FAS and FADD and caspase-3 in oesophagus, stomach and colonic tissues of mice irradiated in vivo by immunohistochemistry. Materials and Methods: A total of 48 adult male C57BL mice were distributed into four groups: Ami(-)/Rad(-): Mice received 0.5 ml of 0.9% physiological saline solution (PPS) intraperitioneally (i.p.); Ami(+)/Rad(-): mice received amifostine (400mg/kg i.p.) freshly dissolved in double-distilled water; Ami(-)/Rad(+): mice received 0.5 ml of PSS i.p. 30 min before a single whole-body radiation dose of 7 Gy; Ami(+)/Rad(+): mice received 0.5 ml of an aqueous solution of 400 mg/kg amifostine i.p.30 min prior to irradiation. All groups were assigned into subgroups sacrificed at 0.5 h, 1 h, 2 h and 4 h after irradiation. Results. In oesophagus and stomach tissues, we did not observe any difference between Ami(-)/ad(-), Ami(+)/Rad(-), Ami(-)/Rad(+) and Ami(+)/Rad(+) groups in the expression of FASL, FAS and FADD. The colonic tissue was the only to exhibit any difference in the expression of FAS and caspase-3 protein in the Ami(-)/Rad(+) group at 1 and 2 h. Amifostine increased FAS and caspase-3 immunoexpression when compared to the control. Immunoexpression for FASL and FADD was not remarkably different in colonic tissue. Conclusion: Taken together, our results demonstrate that amifostine increases FAS and caspase-3 expression in colonic tissue of irradiated mice.
Assunto Amifostine
radiation
mouse
apoptosis
Idioma Inglês
Data 2015-05-01
Publicado em Anticancer Research. Athens: Int Inst Anticancer Research, v. 35, n. 5, p. 2817-2822, 2015.
ISSN 0250-7005 (Sherpa/Romeo, fator de impacto)
Editor Int Inst Anticancer Research
Extensão 2817-2822
Fonte http://ar.iiarjournals.org/content/35/5/2817.abstract
Direito de acesso Acesso restrito
Tipo Artigo
Web of Science WOS:000354267200041
URI http://repositorio.unifesp.br/11600/42604

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