Immunoexpression of DIABLO, AIF and Cytochrome c in Gastric Adenocarcinoma Assessed by Tissue Microarray

Immunoexpression of DIABLO, AIF and Cytochrome c in Gastric Adenocarcinoma Assessed by Tissue Microarray

Author Silva, Larissa Comparini da Autor UNIFESP Google Scholar
Forones, Nora Manoukian Autor UNIFESP Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Ihara, Silvia Sauli Miki Autor UNIFESP Google Scholar
Gomes, Thiago Simao Autor UNIFESP Google Scholar
Artigiani Neto, Ricardo Autor UNIFESP Google Scholar
Oshima, Celina Tizuko Fujiyama Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract The aim of this study was to analyze the immunoexpressioh of (Smac) DIABLO, AIF, cytochrome c, Ki-67 and cleaved caspase-3 in gastric cancer. A tissue microarray (TMA) paraffin block was constructed using gastric adenocarcinoma tissue and adjacent normal adjacent mucosa from 87 patients who had not previously undergone radiotherapy or chemotherapy. Immunohistochemistry was used to evaluate the protein levels. Samples were positive for (Smac) DIABLO in 37 (45.6%) and 37 (46.8%), for AIF in 31 (36.9%) and 36 (45.6%), for cytochrome c in 60 (68.9%) and 44 (54.4%), for Ki-67 in 63 (72.4%) and 52 (61.9%) and for cleaved caspase-3 in 21 (24.1%) and 3 (3.4%) cases of tumor and adjacent normal tissues, respectively. Our results suggest that increased expression of Ki-67 and cleaved caspase-3 could contribute to carcinogenesis. The expression of these proteins indicates an attempt of cells to maintain tissue homeostasis.
Keywords Gastric adenocarcinoma
immunohistochemistry
(Smac) DIABLO
AIF
cytochrome c
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Grant number FAPESP: 2010/15764-8
Date 2013-02-01
Published in Anticancer Research. Athens: Int Inst Anticancer Research, v. 33, n. 2, p. 647-653, 2013.
ISSN 0250-7005 (Sherpa/Romeo, impact factor)
Publisher Int Inst Anticancer Research
Extent 647-653
Origin http://ar.iiarjournals.org/content/33/2/647.long
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000315424000040
URI http://repositorio.unifesp.br/11600/42599

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