MYC Insertions in Diffuse-type Gastric Adenocarcinoma

MYC Insertions in Diffuse-type Gastric Adenocarcinoma

Author Calcagno, Danielle Queiroz Autor UNIFESP Google Scholar
Guimaraes, Adriana Costa Google Scholar
Leal, Mariana Ferreira Autor UNIFESP Google Scholar
Seabra, Aline Damaceno Google Scholar
Khayat, Andre Salim Google Scholar
Pontes, Thais Brilhante Autor UNIFESP Google Scholar
Assumpcao, Paulo Pimentel Autor UNIFESP Google Scholar
Smith, Marilia de Arruda Cardoso Autor UNIFESP Google Scholar
Burbano, Rommel Rodríguez Autor UNIFESP Google Scholar
Institution Fed Univ Para
Universidade Federal de São Paulo (UNIFESP)
Abstract Background: MYC is important in gastric carcinogenesis. A few studies reported MYC translocation or insertion associated with gastric cancer. Materials and Methods: MYC copy number and its insertion, as well as the chromosomes in which MYC was inserted, were evaluated by fluorescence in situ hybridization assay in interphase and metaphase cells of 12 diffuse-type gastric cancer samples. MYC protein expression was evaluated by immunohistochemistry. Results: The presence of 3 MYC signals was the most frequent alteration. All cases also presented 4 and 5 MYC signals. In all samples, we observed chromosome 8 trisomy with MYC copies and MYC insertion into the chromosomes 2, 7, 14, 17, 18 and 22. All samples presented nucleic and cytoplasmic immunoreactivity. Conclusion: MYC cytoplasmic immunoreactivity can be the result of MYC insertion with the breakpoints within or close to the regions that are able to target the nucleus. MYC insertion and cytoplasmatic immunoreactivity may be a common characteristic of diffuse-type gastric cancer.
Keywords Gastric cancer
MYC gene
MYC protein
MYC insertion
hybridization in situ fluorescence
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Date 2009-07-01
Published in Anticancer Research. Athens: Int Inst Anticancer Research, v. 29, n. 7, p. 2479-2483, 2009.
ISSN 0250-7005 (Sherpa/Romeo, impact factor)
Publisher Int Inst Anticancer Research
Extent 2479-2483
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000268104100009

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