Cell proliferation and death in the gastric epithelium of developing rats after glucocorticoid treatments

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dc.contributor.author Gama, Patricia
dc.contributor.author Goldfeder, Eliane Maria [UNIFESP]
dc.contributor.author De Moraes, Julio Cesar Bertoccini
dc.contributor.author Alvares, Eliana Parisi
dc.date.accessioned 2018-06-15T13:50:09Z
dc.date.available 2018-06-15T13:50:09Z
dc.date.issued 2000-11-01
dc.identifier http://dx.doi.org/10.1002/1097-0185(20001101)260:3<213
dc.identifier.citation Anatomical Record. New York: Wiley-liss, v. 260, n. 3, p. 213-221, 2000.
dc.identifier.issn 0003-276X
dc.identifier.uri http://repositorio.unifesp.br/11600/42535
dc.description.abstract Glucocorticoids take part in the intense morphofunctional modifications that occur in the gastric mucosa during fetal and postnatal development. Two studies were designed to evaluate corticoids role in gastric cell proliferation and apoptosis in developing rats: in vivo, using suckling animals; in vitro, using gastric explants obtained from 20-day fetuses. These explants were cultured in DMEM/F12 medium treated or not with 50 ng/ml of corticosterone; after 22 hr, vincristine was added to the medium for 2 hr to block mitosis. The metaphasic index decreased significantly after the 24-hr treatment (controls: 1.52 +/- 0.53; treated: 0.40 +/- 0.21) and apoptotic cells were visualized under light and electron microscopy. Fifteen-day-old rats were treated with hydrocortisone (25 mg/Kg) for 3 days, and injected with BrDU (100 mg/Kg) 1 hr before sacrifice on the 18(th) day. BrDu-labeled and non-labeled cells were counted to determine the labeling index of epithelial cells. As apoptotic cells are rapidly eliminated, other animals were treated for only 2-3 hr. Sections were investigated for the presence of apoptotic cells, using morphological criteria and TUNEL labeling. Hydrocortisone significantly reduced the labeling index (controls: 15.6 +/- 1.6 vs. treated: 11.7 +/- 1.1), besides altering the body weight gain. Hydrocortisone treatment doubled the number of apoptotic cells after 2 hr, and quadruplicated it after 3 hr. The results demonstrated that glucocorticoids inhibit cell proliferation in the gastric epithelium of fetuses and suckling rats and increase apoptotic rates, suggesting the exit from cell cycle. Anat Rec 260: 213-221, 2000. (C) 2000 Wiley-Liss, Inc. en
dc.format.extent 213-221
dc.language.iso eng
dc.publisher Wiley-Blackwell
dc.relation.ispartof Anatomical Record
dc.rights Acesso aberto
dc.subject glucocorticoids en
dc.subject gastric epithelium en
dc.subject cell proliferation en
dc.subject apoptosis en
dc.subject fetus en
dc.subject development en
dc.subject rat en
dc.title Cell proliferation and death in the gastric epithelium of developing rats after glucocorticoid treatments en
dc.type Artigo
dc.rights.license http://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ Sao Paulo, ICB, Dept Histol & Embriol, BR-05508900 Sao Paulo, Brazil
dc.description.affiliation Univ Fed Sao Paulo, Dept Morfol, Sao Paulo, Brazil
dc.description.affiliationUnifesp Univ Fed Sao Paulo, Dept Morfol, Sao Paulo, Brazil
dc.identifier.doi 10.1002/1097-0185(20001101)260:3<213
dc.description.source Web of Science
dc.identifier.wos WOS:000165117400001


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