Quinolone-resistant Escherichia coli

Quinolone-resistant Escherichia coli

Autor Ito, Carmen Antonia Sanches Google Scholar
Gales, Ana Cristina Autor UNIFESP Google Scholar
Tognim, Maria Cristina Bronharo Autor UNIFESP Google Scholar
Munerato, Patricia Autor UNIFESP Google Scholar
Dalla Costa, Libera Maria Google Scholar
Instituição State University of Ponta Grossa
Universidade Federal de São Paulo (UNIFESP)
Federal University of Paraná Hospital de Clínicas Bacteriology Laboratory
Resumo Quinolones (nalidixic acid - NAL, norfloxacin - NOR, ciprofloxacin - CIP and gatifloxacin - GAT) were tested against Escherichia coli isolated from urine (385 patient samples) by disk diffusion (DD) and agar dilution (AD) methods. Fifty-three samples (13.8%) were classified as resistant to at least one of the quinolones tested. CIP and NOR susceptibilities were the same (91.4%) and they were similar to GAT (92.7%). Susceptibility to NAL, detected by the disk diffusion method, was used to predict susceptibility to NOR, CIP and GAT by the agar dilution method. The sensitivity and specificity of NAL were 100% and 95%, respectively. Twelve samples were analyzed for mutations in the quinolone resistance-determining region (QRDR) of the gyrA and parC genes. Sequencing of these genes failed to find any mutations in the quinolone-sensitive isolates. However, three mutations were observed in the isolates resistant to all the quinolones tested - two in gyrA and one in parC. A single mutation in gyrA was found in the strains that were resistant to nalidixic acid but fluoroquinolone-sensitive. These findings support the suggestion that NAL could be used as a marker for susceptibility to fluoroquinolones in routine microbiology laboratories. The overall resistance rate to quinolones in the present study was 13.8%, which is higher than that observed in other studies carried out in developed countries. Our findings serve as a warning that resistance to this group of antimicrobial agents is increasing.
Palavra-chave Urinary infection
quinolone resistance
Escherichia coli
Idioma Inglês
Data de publicação 2008-02-01
Publicado em Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 12, n. 1, p. 5-9, 2008.
ISSN 1413-8670 (Sherpa/Romeo, fator de impacto)
Publicador Brazilian Society of Infectious Diseases
Extensão 5-9
Fonte http://dx.doi.org/10.1590/S1413-86702008000100003
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000257126000003
SciELO S1413-86702008000100003 (estatísticas na SciELO)
Endereço permanente http://repositorio.unifesp.br/handle/11600/4218

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