Ischemic preconditioning attenuates remote pulmonary inflammatory infiltration of diabetic rats with an intestinal and hepatic ischemia-reperfusion injury

Ischemic preconditioning attenuates remote pulmonary inflammatory infiltration of diabetic rats with an intestinal and hepatic ischemia-reperfusion injury

Author Thomaz Neto, Farid Jose Google Scholar
Koike, Marcia Kiyomi Autor UNIFESP Google Scholar
Abrahão, Marcos de Souza Autor UNIFESP Google Scholar
Carillo Neto, Francisco Google Scholar
Pereira, Renan Kenji Hanada Google Scholar
Machado, Jose Lucio Martins Google Scholar
Montero, Edna Frasson de Souza Autor UNIFESP Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
IAMSPE
Abstract PURPOSE: To assess ischemic preconditioning (IPC) effects in pulmonary lesion in intestinal and hepatic ischemia-reperfusion (IR) injury models using diabetic rats.METHODS: Diabetes (DM) was induced in 28 male Wistar rats by alloxan (42 mg/kg, IV). After 28 days, severe DM rats were submitted to intestinal or hepatic IR injury with or without IPC. Intestinal IR (30 min of mesenteric artery occlusion and 30 min of reperfusion; n=6) and IPC groups (10 min ischemia, 10 min reperfusion, followed by intestinal IR; n=6), and Hepatic IR (30 min of hepatic pedicle occlusion and 30 min of reperfusion; n=5) and IPC groups (10 min ischemia, 10 min reperfusion, followed by hepatic IR; n=5), were compared to DM rats group (n=6). Plasmatic lactate, glycemia were measured before and after IR injury. Histomorphology of lung was performed counting inflammatory cells. Data was expressed in mean +/- SE. P<0.05.RESULTS: Glycemia and lactate were similar among groups. IPC did not interfere in these parameters. On histological evaluation, IR increased inflammatory cells infiltration in pulmonary parenchyma compared to control in both IR injury models. IPC attenuated inflammatory infiltration in lungs.CONCLUSION: Ischemic preconditioning protects against remote ischemia-reperfusion injury in lung on intestinal or hepatic ischemia-reperfusion model with acute diabetes.
Keywords Ischemia
Reperfusion
Ischemic Preconditioning
Lung
Diabetes Mellitus
Rats
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 2013-03-01
Published in Acta Cirurgica Brasileira. Sao Paulo: Acta Cirurgica Brasileira, v. 28, n. 3, p. 174-178, 2013.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Acta Cirurgica Brasileira
Extent 174-178
Origin http://dx.doi.org/10.1590/S0102-86502013000300003
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000316126000003
SciELO ID S0102-86502013000300003 (statistics in SciELO)
URI http://repositorio.unifesp.br/11600/41984

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