Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in rats

Effect of N-acetylcysteine on pulmonary cell death in a controlled hemorrhagic shock model in rats

Título alternativo Efeito da N-acetilcisteína na morte celular pulmonar em um modelo de choque hemorrágico controlado em ratos
Autor Saad, Paulo Fernandes Autor UNIFESP Google Scholar
Saad, Karen Ruggeri Autor UNIFESP Google Scholar
Oliveira Filho, Luiz Dantas de Google Scholar
Ferreira, Sueli Gomes Google Scholar
Koike, Marcia Kiyomi Autor UNIFESP Google Scholar
Montero, Edna Frasson de Souza Autor UNIFESP Google Scholar
Instituição Universidade Federal de São Paulo (UNIFESP)
Sao Francisco Valley Fed Univ UNIVASF
Fundacao Univ Fed Vale Sao Francisco
Universidade de São Paulo (USP)
Resumo PURPOSE: To evaluate the effect of N-acetylcysteine (NAC) combined with fluid resuscitation on pulmonary cell death in rats induced with controlled hemorrhagic shock (HS).METHODS: Two arteries (MAP calculation and exsanguination) and one vein (treatments) were catheterized in 22 anesthetized rats. Two groups of male albino rats were induced with controlled HS at 35mmHg MAP for 60 min. After this period, the RL group was resuscitated with Ringer's lactate and the RL+NAC group was resuscitated with Ringer's lactate combined with 150mg/Kg NAC. The control group animals were cannulated only. The animals were euthanized after 120 min of fluid resuscitation. Lung tissue samples were collected to evaluate the following: histopathology, TUNEL and imunohistochemical expression of caspase 3.RESULTS: RL showed a greater number of cells stained by TUNEL than RL + NAC, but there was no change in caspase 3 expression in any group.CONCLUSION: N-acetylcysteine associate to fluid resuscitation, after hemorrhagic shock, decreased cell death attenuating lung injury.
Assunto Acetylcysteine
Lung
Shock, Hemorrhagic
Cell Death
Rats
Idioma Inglês
Financiador Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Data 2012-08-01
Publicado em Acta Cirurgica Brasileira. Sao Paulo: Acta Cirurgica Brasileira, v. 27, n. 8, p. 561-565, 2012.
ISSN 0102-8650 (Sherpa/Romeo, fator de impacto)
Editor Acta Cirurgica Brasileira
Extensão 561-565
Fonte http://dx.doi.org/10.1590/S0102-86502012000800008
Direito de acesso Acesso aberto Open Access
Tipo Artigo
Web of Science WOS:000306972600008
SciELO S0102-86502012000800008 (estatísticas na SciELO)
URI http://repositorio.unifesp.br/11600/41982

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