Proliferation of the superficial epithelium of ovaries in senile female rats following oral administration of conjugated equine estrogens

Proliferation of the superficial epithelium of ovaries in senile female rats following oral administration of conjugated equine estrogens

Author Perniconi, Sergio Eduardo Autor UNIFESP Google Scholar
Simões, Manuel de Jesus Autor UNIFESP Google Scholar
Simões, Ricardo dos Santos Google Scholar
Haidar, Mauro Abi Autor UNIFESP Google Scholar
Baracat, Edmund Chada Autor UNIFESP Google Scholar
Soares Júnior, José Maria Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract OBJECTIVE: To evaluate the effect of different concentrations of estrogen on the ovarian superficial epithelium in senile female rats. Design: Fifty female rats at 15 months of age and with irregular estrous cycles were selected and randomly divided into five experimental groups containing equal numbers of animals in each: GPROP, control group receiving vehicle only; GE0.05mg, group receiving conjugated equine estrogens (CEE) at a dose of 50 µg/kg; GE0.5mg, group receiving CEE at 500 µg/kg; GE1mg, group receiving CEE at 1 mg/kg; and GE2mg, receiving CEE at 2 mg/kg. The length of treatment was 21 days. After this period, the animals were anesthetized and the ovaries were fixed in 10% formaldehyde and processed for routine histology. Histomorphology was analyzed by light microscopy, and histomorphometrics were evaluated using the Imagelab program. RESULTS: In the GPROP and GE0.05mg groups, the superficial epithelium of the ovary had a simple cuboidal shape, and as the estrogen dose increased, the epithelium thickened, with pseudo-stratified or stratified epithelium appearing in the GE2mg group. The animals in the group given the highest estrogen dose (GE2mg) showed the thickest ovarian epithelium and the largest perimeter and surface area of the surface ovarian epithelium (P < 0.01). However, the difference in epithelium thickness between the GE0.5mg and GE1mg groups was only slight. CONCLUSION: Our data suggest that CEE at a dose of 2 mg/kg may induce marked proliferation of rat ovarian epithelium.
Keywords Estrogens
Superficial epithelium
Ovary
Animal Model
Language English
Date 2008-01-01
Published in Clinics. Faculdade de Medicina / USP, v. 63, n. 3, p. 381-388, 2008.
ISSN 1807-5932 (Sherpa/Romeo, impact factor)
Publisher Faculdade de Medicina / USP
Extent 381-388
Origin http://dx.doi.org/10.1590/S1807-59322008000300016
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000256919600016
SciELO ID S1807-59322008000300016 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/4164

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