Experimental model of severe acute pancreatitis in rabbits

Experimental model of severe acute pancreatitis in rabbits

Alternative title Modelo experimental de pancreatite aguda grave em coelhos
Author Goldenberg, Alberto Autor UNIFESP Google Scholar
Romeo, Ana Celia Diniz Cabral Barbosa Autor UNIFESP Google Scholar
Moreira, Márcia Bento Google Scholar
Apodaca-Torrez, Franz Robert Autor UNIFESP Google Scholar
Linhares, Marcelo Moura Autor UNIFESP Google Scholar
Matone, Jacques Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
UNIBAN Department of Surgery
Cruzeiro do Sul University
Abstract PURPOSE: To develop an experimental model of severe acute pancreatitis in rabbits through a pancreatic ductal injection of sodium taurocholate. METHODS: Twenty-four albino rabbits of the New Zealand lineage were distributed into four groups of six animals (A, B, C and S). The rabbits of three experimental groups (A, B and C) were submitted to a laparatomy and received a pancreatic ductal injection of 1ml/kg sodium taurocholate 5%. Also, they were submitted to further laparatomies after 4h, 8h and 12h, respectively. The control group (S) was subdivided into two groups of three animals: in subgroup S1 only the pancreatic duct catheterization was performed whereas in subgroup S2 the pancreatic duct catheterization as well as an injection of 1ml/kg physiologic solution 0.9% were carried out. After 12 hours, the rabbits were evaluated. In the re-intervention, blood was collected to determine the amylasemia and a pancreatectomy was carried out to investigate interstitial infiltration, steatonecrosis and necrosis of the organ, using an optical microscope. RESULTS: There was an elevation of amylase in all groups thus proving the existence of acute pancreatitis. The size of the interlobular septum increased progressively with a greater variation between group S1 (0.13) and group C (0. 53) (p=0.035). While all the animals in group A exhibited focal cellular necrosis, it was more intense in the rabbits of group B and culminated with a high proportion of severe pancreatic necrosis in group C animals. The difference in the intensity of cellular necrosis showed statistic significance (p=0.001). CONCLUSION: The proposed experimental model demonstrated its reproducibility and effectiveness in producing severe acute pancreatitis in rabbits.

OBJETIVO: Desenvolver modelo experimental de pancreatite aguda grave em coelhos por meio da injeção de taurocolato de sódio no ducto pancreático. MÉTODOS: Vinte e quatro coelhos albinos da linhagem Nova Zelândia foram distribuídos em quatro grupos de seis animais (A, B, C e S). Os coelhos dos três grupos experimentais (A, B e C) foram submetidos a laparotomia e injetou-se taurocolato de sódio a 5%, 1ml/Kg no ducto pancreático. Realizou-se nova laparotomia, respectivamente, após 4h, 8h e 12h. No grupo controle (S), subdividido em dois grupos de três animais, foi realizada no subgrupo S1 apenas cateterização do ducto pancreático e no subgrupo S2 cateterização do ducto pancreático e injeção de solução fisiológica 0,9%, 1ml/Kg. Estes animais foram reavaliados após 12 horas. Na reintervenção coletou-se sangue para determinação da amilasemia e realizou-se pancreatectomia para análise histológica do infiltrado intersticial, da esteatonecrose e da necrose do órgão. RESULTADOS: Houve elevação da amilase em todos os grupos, demonstrando a presença da pancreatite aguda. O tamanho do septo interlobular aumentou progressivamente, observando-se maior diferença entre os grupos S1 (0,13) e C (0,53) (p=0,035). Todos os animais do grupo A apresentaram necrose celular focal que se tornou mais intensa nos coelhos do grupo B, culminando com o predomínio de necrose pancreática acentuada nos animais do grupo C. A diferença na intensidade da necrose celular apresentou significância estatística (p=0,001). CONCLUSÃO: O modelo experimental proposto se mostrou reprodutível e efetivo em provocar pancreatite aguda grave em coelhos.
Keywords Pancreatitis, Acute Necrotizing
Animal Experimentation
Rabbits
Pancreatite Necrosante Aguda
Experimentação Animal
Coelhos
Language English
Date 2007-10-01
Published in Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 22, n. 5, p. 366-371, 2007.
ISSN 0102-8650 (Sherpa/Romeo, impact factor)
Publisher Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Extent 366-371
Origin http://dx.doi.org/10.1590/S0102-86502007000500008
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000258573400009
SciELO ID S0102-86502007000500008 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/3950

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