B-1 cells produce insulin and abrogate experimental streptozotocin-induced diabetes

B-1 cells produce insulin and abrogate experimental streptozotocin-induced diabetes

Author Alvares-Saraiva, Anuska M. Autor UNIFESP Google Scholar
Novo, Marilia C. T. Autor UNIFESP Google Scholar
Oliveira, Vivian Cristina de Autor UNIFESP Google Scholar
Maricato, Juliana Terzi Autor UNIFESP Google Scholar
Lopes, Jose Daniel Autor UNIFESP Google Scholar
Popi, Ana Flavia Autor UNIFESP Google Scholar
Mariano, Mario Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Paulista UNIP
Abstract The participation of B-1 cells in a murine model of spontaneous diabetes has been recently reported. Here, we describe the role of B-1 cells in streptozotocin (STZ) induced diabetes in mice. We demonstrated that XID (B-1 cell-deficient) mice are more susceptible to STZ treatment than WT mice, as evidenced by their higher blood glucose level in response to STZ. Unexpectedly, the XID mice that were i.p. transferred with purified B-1 cells, either before or after the STZ treatment, did not develop diabetes. These cell transfers provided long-lasting protection for the XID mice against STZ-induced diabetes, suggesting that B-1 cells play an important role in the experimental diabetes pathobiology. We also showed that B-1 cell culture supernatants were able to regulate the blood glucose level of the diabetic XID mice, and we identified insulin-producing cells when B-1 cells were differentiated in B-1 cell-derived phagocyte in vitro. These findings provide a novel role for B-1 cells in metabolic processes, presenting a new mechanism to explain the pathogenesis of diabetes and a possible therapeutical target.
Keywords B-1 cells
Experimental diabetes
Insulin
Streptozotocin
XID mice
Language English
Sponsor Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Grant number CNPq: 143031/2008-2
FAPESP: 2008/58561-0
FAPESP: 2011/50256-6
Date 2015-05-01
Published in European Journal of Immunology. Hoboken: Wiley-Blackwell, v. 45, n. 5, p. 1452-1461, 2015.
ISSN 0014-2980 (Sherpa/Romeo, impact factor)
Publisher Wiley-Blackwell
Extent 1452-1461
Origin http://dx.doi.org/10.1002/eji.201445409
Access rights Closed access
Type Article
Web of Science ID WOS:000354182300017
URI http://repositorio.unifesp.br/handle/11600/39024

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