Adipose tissue-derived stem cell therapy in rat cryopreserved ovarian grafts

Adipose tissue-derived stem cell therapy in rat cryopreserved ovarian grafts

Author Damous, Luciana Lamarão Autor UNIFESP Google Scholar
Nakamuta, Juliana Sanajotti Google Scholar
Carvalho, Ana Elisa Teofilo Saturi de Google Scholar
Soares Junior, Jose Maria Google Scholar
Simoes, Manuel de Jesus Autor UNIFESP Google Scholar
Krieger, Jose Eduardo Google Scholar
Baracat, Edmund Chada Autor UNIFESP Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract The preliminary results of ovarian transplantation in clinical practice are encouraging. However, the follicular depletion caused by ischemic injury is a main concern and is directly related to short-term graft survival. Cell therapy with adipose tissue-derived stem cells (ASCs) could be an alternative to induce early angiogenesis in the graft. This study aimed to evaluate ASCs therapy in rat cryopreserved ovarian grafts. A single dose of rat ASC (rASCs) or vehicle was injected into the bilateral cryopreserved ovaries of twelve adult female rats immediately after an autologous transplant. Daily vaginal smears were performed for estrous cycle evaluation until euthanasia on postoperative day 30. Follicle viability, graft morphology and apoptosis were assessed. No differences were found with respect to estrous cycle resumption and follicle viability (P > 0.05). However, compared with the vehicle-treated grafts, the morphology of the ASCs-treated grafts was impaired, with diffuse atrophy and increased apoptosis (P < 0.05). ASCs direct injected in the stroma of rat cryopreserved ovarian grafts impaired its morphology although may not interfere with the functional resumption on short-term. Further investigations are necessary to evaluated whether it could compromise their viability in the long-term.
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Grant number FAPESP: 2010/17897-5
FAPESP: 2012/09469-9
Date 2015-04-15
Published in Stem Cell Research & Therapy. London: Biomed Central Ltd, v. 6, 6 p., 2015.
ISSN 1757-6512 (Sherpa/Romeo, impact factor)
Publisher Biomed Central Ltd
Extent 6
Origin http://dx.doi.org/10.1186/s13287-015-0068-3
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000353759400001
URI http://repositorio.unifesp.br/handle/11600/39002

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