Aerobic exercise training improves oxidative stress and ubiquitin proteasome system activity in heart of spontaneously hypertensive rats

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dc.contributor.author Soares de Andrade, Luiz Henrique [UNIFESP]
dc.contributor.author Almeida Monteiro de Moraes, Wilson Max [UNIFESP]
dc.contributor.author Matsuo Junior, Eduardo Hiroshi [UNIFESP]
dc.contributor.author Carvalho de Moura, Elizabeth de Orleans [UNIFESP]
dc.contributor.author Moreira Antunes, Hanna Karen [UNIFESP]
dc.contributor.author Montemor, Jairo [UNIFESP]
dc.contributor.author Antonio, Ednei Luiz [UNIFESP]
dc.contributor.author Bocalini, Danilo Sales [UNIFESP]
dc.contributor.author Serra, Andrey Jorge [UNIFESP]
dc.contributor.author Ferreira Tucci, Paulo Jose [UNIFESP]
dc.contributor.author Brum, Patricia Chakur
dc.contributor.author Medeiros, Alessandra [UNIFESP]
dc.date.accessioned 2016-01-24T14:40:18Z
dc.date.available 2016-01-24T14:40:18Z
dc.date.issued 2015-04-01
dc.identifier http://dx.doi.org/10.1007/s11010-015-2326-1
dc.identifier.citation Molecular and Cellular Biochemistry. Dordrecht: Springer, v. 402, n. 1-2, p. 193-202, 2015.
dc.identifier.issn 0300-8177
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/38937
dc.description.abstract The activity of the ubiquitin proteasome system (UPS) and the level of oxidative stress contribute to the transition from compensated cardiac hypertrophy to heart failure in hypertension. Moreover, aerobic exercise training (AET) is an important therapy for the treatment of hypertension, but its effects on the UPS are not completely known. the aim of this study was to evaluate the effect of AET on UPS's activity and oxidative stress level in heart of spontaneously hypertensive rats (SHR). A total of 53 Wi-star and SHR rats were randomly divided into sedentary and trained groups. the AET protocol was 59/week in treadmill for 13 weeks. Exercise tolerance test, non-invasive blood pressure measurement, echocardiographic analyses, and left ventricle hemodynamics were performed during experimental period. the expression of ubiquitinated proteins, 4-hydroxynonenal (4-HNE), Akt, phospho-Akt(ser473), GSK3 beta, and phospho-GSK3 beta(ser9) were analyzed by western blotting. the evaluation of lipid hydroperoxide concentration was performed using the xylenol orange method, and the proteasomal chymotrypsin-like activity was measured by fluorimetric assay. Sedentary hypertensive group presented cardiac hypertrophy, unaltered expression of total Akt, phospho-Akt, total GSK3 beta and phospho-GSK3 beta, UPS hyperactivity, increased lipid hydroperoxidation as well as elevated expression of 4-HNE but normal cardiac function. in contrast, AET significantly increased exercise tolerance, decreased resting systolic blood pressure and heart rate in hypertensive animals. in addition, the AET increased phospho-Akt expression, decreased phospho-GSK3 beta, and did not alter the expression of total Akt, total GSK3 beta, and ubiquitinated proteins, however, significantly attenuated 4-HNE levels, lipid hydroperoxidation, and UPS's activity toward normotensive group levels. Our results provide evidence for the main effect of AET on attenuating cardiac ubiquitin proteasome hyperactivity and oxidative stress in SHR rats. en
dc.description.sponsorship Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorship Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent 193-202
dc.language.iso eng
dc.publisher Springer
dc.relation.ispartof Molecular and Cellular Biochemistry
dc.rights Acesso restrito
dc.subject Hypertension en
dc.subject Aerobic exercise training en
dc.subject Cardiac remodeling en
dc.subject Ubiquitin proteasome system en
dc.subject Oxidative stress en
dc.title Aerobic exercise training improves oxidative stress and ubiquitin proteasome system activity in heart of spontaneously hypertensive rats en
dc.type Artigo
dc.rights.license http://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Univ Sao Judas Tadeu
dc.contributor.institution Univ Nove Julho
dc.contributor.institution Universidade de São Paulo (USP)
dc.description.affiliation Universidade Federal de São Paulo, Dept Biociencias, Silva Jardim, BR-11015020 Santos, SP, Brazil
dc.description.affiliation Universidade Federal de São Paulo, Cardiophysiol & Pathophysiol Lab, São Paulo, Brazil
dc.description.affiliation Univ Sao Judas Tadeu, Dept Postgrad Phys Educ, São Paulo, Brazil
dc.description.affiliation Univ Nove Julho, Postgrad Program Biophoton Appl Hlth Sci, São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Sch Phys Educ & Sport, São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Biociencias, Silva Jardim, BR-11015020 Santos, SP, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Cardiophysiol & Pathophysiol Lab, São Paulo, Brazil
dc.description.sponsorshipID CNPq: 474085/2011-2
dc.identifier.doi 10.1007/s11010-015-2326-1
dc.description.source Web of Science
dc.identifier.wos WOS:000352849800019



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